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Characterization and in vitro evaluation of bacterial cellulose membranes functionalized with osteogenic growth peptide for bone tissue engineering

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Abstract

The aim of this study was to characterize the physicochemical properties of bacterial cellulose (BC) membranes functionalized with osteogenic growth peptide (OGP) and its C-terminal pentapeptide OGP[10–14], and to evaluate in vitro osteoinductive potential in early osteogenesis, besides, to evaluate cytotoxic, genotoxic and/or mutagenic effects. Peptide incorporation into the BC membranes did not change the morphology of BC nanofibers and BC crystallinity pattern. The characterization was complemented by Raman scattering, swelling ratio and mechanical tests. In vitro assays demonstrated no cytotoxic, genotoxic or mutagenic effects for any of the studied BC membranes. Culture with osteogenic cells revealed no difference in cell morphology among all the membranes tested. Cell viability/proliferation, total protein content, alkaline phosphatase activity and mineralization assays indicated that BC-OGP membranes enabled the highest development of the osteoblastic phenotype in vitro. In conclusion, the negative results of cytotoxicity, genotoxicity and mutagenicity indicated that all the membranes can be employed for medical supplies, mainly in bone tissue engineering/regeneration, due to their osteoinductive properties.

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Acknowledgments

Financial support from the Brazilian agencies FAPESP (grant numbers: 08-58776-6 and 09/09960-1) and CNPq is acknowledged. The authors wish to thank Prof. Dr. Luiz Geraldo Vaz of Department of Dental Materials and Prosthodontics, Dental School at Araraquara—UNESP for support in the mechanical tests and Mr. Roger Rodrigues Fernandes for technical support.

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Authors and Affiliations

  1. Institute of Chemistry, Universidade Estadual Paulista, (UNESP), Rua Francisco Degni 55, Araraquara, SP, 14800-900, Brazil

    Sybele Saska, Younès Messaddeq, Sidney José Lima Ribeiro & Reinaldo Marchetto

  2. Department of Morphology, Dental School at Araraquara, Universidade Estadual Paulista, (UNESP), Rua Humaitá, 1680, Araraquara, SP, 14801-903, Brazil

    Raquel Mantuaneli Scarel-Caminaga & Ana Maria Minarelli Gaspar

  3. Cell Culture Laboratory, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, (USP), Av. do Café s/n, Ribeirão Preto, SP, 14040-904, Brazil

    Lucas Novaes Teixeira, Paulo Tambasco de Oliveira & Adalberto Luiz Rosa

  4. Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, (USP), Bloco G, Av. Bandeirantes, Ribeirão Preto, SP, 3900, Brazil

    Leonardo Pereira Franchi, Raquel Alves dos Santos & Catarina Satie Takahashi

  5. Department of Biology, Faculty of Philosophy Sciences and Letters of Ribeirão Preto, University of São Paulo, (USP), Av. Bandeirantes, Ribeirão Preto, SP, 3900, Brazil

    Catarina Satie Takahashi

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  1. Sybele Saska
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Correspondence to Sybele Saska.

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Saska, S., Scarel-Caminaga, R.M., Teixeira, L.N. et al. Characterization and in vitro evaluation of bacterial cellulose membranes functionalized with osteogenic growth peptide for bone tissue engineering. J Mater Sci: Mater Med 23, 2253–2266 (2012). https://doi.org/10.1007/s10856-012-4676-5

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