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In vitro behavior of layer-by-layer deposited molecular oligoelectrolyte films on Ti–6Al–4V surfaces

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Abstract

Layer-by-layer self-assembled films of molecular oligoelectrolytes were used to modify Ti–6Al–4V surfaces in order to test their ability as potential drug delivery system. With regard to medical application the in vitro behavior of the modified material was investigated. The Ti–6Al–4V (6% aluminium, 4% vanadium) material was treated in a layer-by-layer (LbL) process with 2, 4, 6 and 8 layers of molecular oligoelectrolytes 1 and 2 and thereby doped with a fluorescent reporter molecule 2. Human osteoblasts were cultured for a period up to 5 days on the modified material. Ti–6Al–4V surfaces without modification were used as control. In order to investigate the in vitro behavior of the coating as well as the influence of components of the coating on osteoblastic cells, respectively, cell proliferation, differentiation and attachment of hFOB cells were observed by means of cell number, osteoblastic gene expression and fluorescence microscopy. Degradation behavior of the OEM (oligoelectrolyte multilayer film) was examined using optical spectroscopy. Measurement data imply that the layer-by-layer coating was successfully assembled on the Ti surface and endures steam sterilization. The fluorescence signal in cell culture medium increased strictly linear with increasing pre-assembled number of layers on the surface. Proliferation rates of the cells in experimental groups did not differ significantly from each other (P ≥ 0.783). Differentiation pattern was not significantly changed by the coating. The fluorescent reporter component of the film was absorbed by osteoblastic cells and was detected by fluorescence microscopy.

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Acknowledgements

The investigations were supported by the DFG GZ: NE 339/5-1 and Hi 468/16-1. Cells were kindly provided by the Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Würzburg, Germany. The authors gratefully acknowledge the excellent technical assistance of Ms. Andrea Krautheim-Zenk and the likeable support of Mr. Peter Heinl in scanning electron microscopy.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuremberg, Glückstraße 11, 91054, Erlangen, Germany

    Sabine Ponader, Cornelius von Wilmowsky, Karl A. Schlegel, Friedrich W. Neukam & Emeka Nkenke

  2. Department of Chemistry and Pharmacy and Interdisciplinary Center for Molecular Materials (ICMM), University of Erlangen-Nuremberg, Henkestraße 42, 91054, Erlangen, Germany

    Karin Rosenlehner, Cordula D. Schmidt, Torsten Schunk & Andreas Hirsch

  3. Department of Oral and Maxillofacial Surgery, University of Athens Medical School, Vas. Sofias 93 and Dim. Soutsou 1, 11521, Athens, Greece

    Eleftherios Vairaktaris

Authors
  1. Sabine Ponader
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  2. Karin Rosenlehner
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  3. Eleftherios Vairaktaris
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  4. Cornelius von Wilmowsky
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  5. Karl A. Schlegel
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  6. Friedrich W. Neukam
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  7. Cordula D. Schmidt
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  8. Torsten Schunk
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  9. Andreas Hirsch
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  10. Emeka Nkenke
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Correspondence to Sabine Ponader.

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Sabine Ponader and Karin Rosenlehner have contributed equally to this work.

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Ponader, S., Rosenlehner, K., Vairaktaris, E. et al. In vitro behavior of layer-by-layer deposited molecular oligoelectrolyte films on Ti–6Al–4V surfaces. J Mater Sci: Mater Med 20, 2455–2463 (2009). https://doi.org/10.1007/s10856-009-3825-y

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  • DOI: https://doi.org/10.1007/s10856-009-3825-y

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