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Cell supports of chitosan/hyaluronic acid and chondroitin sulphate systems. Morphology and biological behaviour

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Abstract

Films and sponges of chitosan (CHI), chitosan/hyaluronic acid (CHI–HA) and chitosan/chondroitin sulphate (CHI–CHOS), were prepared by film deposition or lyophilization (sponges), avoiding the formation of interpolyelectrolyte complexes. The biological behaviour of the systems was analysed by studying the cell behaviour using a fibroblast cell line and standard biological MTT and Alamar Blue tests. The morphology of films, sponges and cell seeded samples was analysed by ESEM. The results obtained indicate that all the systems can be considered as good supports for cell adhesion and proliferation, but there is specific activation of the proliferative process in the presence of hyaluronic acid and chondroitin sulphate.

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Acknowledgements

The authors acknowledge to IDEBIO and BIOIBERICA companies for providing the samples of CHI, hyaluronic acid and chondroitin sulphate. Support from CYTED and CICYT grants is also acknowledged. This work has been carried out in the framework of the NoE EXPERTISSUES. Mar Fernández is supported by the Ramón y Cajal Program.

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Authors and Affiliations

  1. Centro de Biomateriales, Universidad de La Habana, La Habana, 10400, Cuba

    Carlos Peniche

  2. Departamento de Farmacología, Facultad de Farmacia, UCM, Madrid, Spain

    Mar Fernández

  3. Hospital Provincial de Ávila, c/ Jesús del Gran Poder, s/n, Avila, Spain

    Juan Parra, Javier Jimenez & Antonio López Bravo

  4. Instituto de Ciencia y Tecnología de Polímeros, CSIC, Juan de la Cierva 3, Madrid, 28006, Spain

    Gema Rodríguez, David Gómez & Julio San Román

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  1. Carlos Peniche
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  2. Mar Fernández
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  3. Gema Rodríguez
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  4. Juan Parra
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  5. Javier Jimenez
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  6. Antonio López Bravo
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  7. David Gómez
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  8. Julio San Román
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Correspondence to Gema Rodríguez.

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Peniche, C., Fernández, M., Rodríguez, G. et al. Cell supports of chitosan/hyaluronic acid and chondroitin sulphate systems. Morphology and biological behaviour. J Mater Sci: Mater Med 18, 1719–1726 (2007). https://doi.org/10.1007/s10856-007-3032-7

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  • DOI: https://doi.org/10.1007/s10856-007-3032-7

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