Abstract
Purpose
To assess the impact of peritoneal endometriosis on oocyte and embryo quality in a mouse model.
Methods
Peritoneal endometriosis was surgically induced in 33 B6CBA/F1 female mice (endometriosis group, N = 17) and sham-operated were used as control (sham group, N = 16). Mice were superovulated 4 weeks after surgery and mated or not, to collect E0.5-embryos or MII-oocytes. Evaluation of oocyte and zygote quality was done by immunofluorescence under spinning disk confocal microscopy.
Results
Endometriosis-like lesions were observed in all mice of endometriosis group. In both groups, a similar mean number of MII oocytes per mouse was observed in non-mated mice (30.2 vs 32.6), with a lower proportion of normal oocytes in the endometriosis group (61 vs 83 %, p < 0.0001). Abnormalities were incomplete extrusion or division of the first polar body and spindle abnormalities. The mean number of zygotes per mouse was lower in the endometriosis group (21 vs 35.5, p = 0.02) without difference in embryo quality.
Conclusions
Our results support that induced peritoneal endometriosis in a mouse model is associated with a decrease in oocyte quality and embryo number. This experimental model allows further studies to understand mechanisms of endometriosis-associated infertility.
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Acknowledgments
We thank Romain Morichon for the use of the spinning disk confocal microscopy, Michele Oster for the histology, Lauriane Roche for her help in building the figures.
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Capsule In a mouse model, peritoneal endometriosis was responsible for decrease in oocyte quality and embryo quantity. Number of ovulated oocytes was not impaired.
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Cohen, J., Ziyyat, A., Naoura, I. et al. Effect of induced peritoneal endometriosis on oocyte and embryo quality in a mouse model. J Assist Reprod Genet 32, 263–270 (2015). https://doi.org/10.1007/s10815-014-0390-1
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DOI: https://doi.org/10.1007/s10815-014-0390-1