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Peritoneal Fluid From Infertile Women With Minimal/Mild Endometriosis Compromises the Meiotic Spindle of Metaphase II Bovine Oocytes: A Pilot Study

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Abstract

Some studies have demonstrated alterations in the composition of peritoneal fluid (PF) from women with endometriosis. Controversial studies have suggested that impaired oocyte quality may be involved in the pathogenesis of endometriosis-related infertility. The aim of this study was to evaluate the spindle and chromosome distribution of in vitro-matured oocytes in the presence of 2 concentrations of PF from infertile women with minimal/mild endometriosis (EI/II) compared to fertile controls. We performed an experimental study using a bovine model. Samples of PF were obtained from 12 women who underwent video-laparoscopy—6 infertile women with EI/II and 6 fertile women without endometriosis (control group). Immature bovine oocytes underwent in vitro maturation (IVM) in the absence of PF and in the presence of 2 concentrations (1% and 10%) of PF from fertile women and from infertile women with EI/II. After 22 to 24 hours of IVM, oocytes were fixed for subsequent immunofluorescence staining for the visualization of microtubules and chromosomes by confocal microscopy. The percentage of meiotically normal oocytes was significantly lower for oocytes that underwent IVM in the presence of 1% (62.50%) and 10% (56.25%) of PF from infertile women with EI/II than in the absence of PF (88.46%) and in the presence of 1% (78.57%) and 10% (84.61%) of PF from fertile women (P <.01). We demonstrated that PF from infertile women with EI/II promotes meiotic abnormalities in in vitro- matured bovine oocytes. Therefore, our results contribute to the understanding of the etiopathogenic mechanisms of infertility related to EI/II.

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Authors and Affiliations

  1. Human Reproduction Division, Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900, Ribeirao Preto, São Paulo, Brazil

    Bruna Talita Gazeto Melo Jianini MSc, Vanessa Silvestre Innocenti Giorgi MSc, Júlio César Rosa e Silva MD, PhD, Rui Alberto Ferriani MD, PhD & Paula Andrea Navarro MD, PhD

  2. Department of Genetics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Michele Gomes Da Broi PhD & Cláudia Cristina Paro de Paz PhD

  3. National Institute of Hormones and Woman’s Health, CNPq, Porto Alegre, Rio Grande do Sul, Brazil

    Rui Alberto Ferriani MD, PhD & Paula Andrea Navarro MD, PhD

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  1. Bruna Talita Gazeto Melo Jianini MSc
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  2. Vanessa Silvestre Innocenti Giorgi MSc
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  3. Michele Gomes Da Broi PhD
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  4. Cláudia Cristina Paro de Paz PhD
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  5. Júlio César Rosa e Silva MD, PhD
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  6. Rui Alberto Ferriani MD, PhD
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  7. Paula Andrea Navarro MD, PhD
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Correspondence to Bruna Talita Gazeto Melo Jianini MSc.

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Jianini, B.T.G.M., Giorgi, V.S.I., Da Broi, M.G. et al. Peritoneal Fluid From Infertile Women With Minimal/Mild Endometriosis Compromises the Meiotic Spindle of Metaphase II Bovine Oocytes: A Pilot Study. Reprod. Sci. 24, 1304–1311 (2017). https://doi.org/10.1177/1933719116687658

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