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Different Effects of Forsythoside E on Acetylcholinesterase and Butyrylcholinesterase by Fluorescent Spectroscopy

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Journal of Applied Spectroscopy Aims and scope

Forsythoside E is one of the major secondary metabolites in Forsythia suspensa. The interactions between forsythoside E and two types of cholinesterases, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were investigated in PBS buffer (pH 7.40) by using multispectroscopic techniques. Forsythoside E increased the fluorescence intensity of AChE but quenched the fluorescence of BChE. Synchronous fluorescence studies showed that forsythoside E mainly acts on tyrosine residues of AChE and tryptophan residues of BChE. It was also proved that the complex between the compound and cholinesterases formed spontaneously at a stoichiometric ratio of 1:1 via multispectral technology. Finally, forsythoside E inhibited the activities of cholinesterases with similar IC50 values of 1.08 mM for AChE and 0.92 mM for BChE. The results illuminate the details of the interaction between forsythoside E and cholinesterases.

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Authors and Affiliations

  1. Institute of Molecular Science, Shanxi University, Taiyuan, China

    Ch. Gao & H. Du

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  1. Ch. Gao
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  2. H. Du
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Correspondence to H. Du.

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Abstract of article is published in Zhurnal Prikladnoi Spektroskopii, Vol. 89, No. 3, p. 432, May–June, 2022.

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Gao, C., Du, H. Different Effects of Forsythoside E on Acetylcholinesterase and Butyrylcholinesterase by Fluorescent Spectroscopy. J Appl Spectrosc 89, 559–566 (2022). https://doi.org/10.1007/s10812-022-01395-5

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