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Phenolic Hydroxyl Group–Carbon Dots as a Fluorescent Probe for the Detection of Hydrogen Peroxide and Glucose in Milk

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Journal of Applied Spectroscopy Aims and scope

In order to directly detect the concentration of glucose in milk, carbon dots (CDs) rich in phenolic hydroxyl groups were synthesized in one step as a fluorescent probe. We presented a new glucose oxidase (GOx)-mediated strategy to detect glucose, which allowed the quantitative analysis of hydrogen peroxide (H2O2) and glucose. Furthermore, it was possible to detect H2O2 and glucose directly in complex systems such as milk. The result in milk showed that the fluorescence of the CDs was quenched by H2O2 with the concentration range from 1 to 100 μM linearly, and the correlation coefficient was 0.977 with a detection limit of 0.175 μM. Similarly, a linear correlation was built between the fluorescence of the CDs and the concentration of glucose in the range from 10 to 100 μM with a correlation coefficient of 0.968 and a detection limit of 0.686 μM. The recovery rate was 97.30–101.05%, which showed high sensitivity in the detection of glucose in milk. As far as we know, this was the first time that CDs were used as a fluorescent probe to detect glucose in milk directly, which removed the step of pretreating milk and provided a supplement and extension for the detection of glucose in fluorescent spectroscopy.

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References

  1. A. Galant, R. Kaufman, and J. Wilson, Food Chem., 188, 149–160 (2015).

    Article  Google Scholar 

  2. S. Kiran and R. Misra, J. Biomed. Mater. Res. A, 103, 2888–2897 (2015).

    Article  Google Scholar 

  3. C. F. Nascimento, P. M. Santos, E. R. Pereira-Filho, and F. R. P. Rocha, Food Chem., 221, 1232–1244 (2017).

    Article  Google Scholar 

  4. Y. Wu and Y. Zhang, Food & Chem. Toxic., 56, 325–335 (2013).

    Article  Google Scholar 

  5. J. Ma, X. Hou, B. Zhang, Y. Wang, and L. He, J. Pharm. Biomed. Anal., 91, 24–31 (2014).

    Article  Google Scholar 

  6. S. Carballo, F. A. Zingarello, S. E. Maestre, J. L. Todolí, and M. S. Prats, Int. J. Food Sci. Technol., 49, 146–152 (2014).

    Article  Google Scholar 

  7. M. P. Gangola, S. Jaiswal, Y. P. Khedikar, and R. N. Chibbar, Food Chem., 154, 127–133 (2014).

    Article  Google Scholar 

  8. Q. Zhao, C. Zhou, Q. Yang, Z. Chu, and N. Jia, Microchim. Acta, 186, 1–8 (2019).

    Article  ADS  Google Scholar 

  9. C. Peng, C. Liu, and Z. Xie, Anal. Methods, 7, 9749–9752 (2015).

    Article  Google Scholar 

  10. S. Kiran and R. D. K. Misra, Mater. Technol., 30, Article ID 1753555714Y.000 (2015).

  11. Y. Ngo, W. M. Choi, J. S. Chung, and S. H. Hur, Sensors Act., B282, 36–44 (2019).

  12. P. Shen and Y. Xia, Anal. Chem., 86, 5323–5329 (2014).

    Article  Google Scholar 

  13. S. T. Yang, L. Cao, P. G. Luo, F. Lu, and Y. P. Sun, J. Am. Chem. Soc., 131, 11308–11309 (2009).

    Article  Google Scholar 

  14. A. Iqbal, K. Iqbal, L. Xu, B. Li, D. Gong, X. Liu, Y. Guo, W. Liu, W. Qin, and H. Guo, Sensors Act. B: Chem., 255, 1130–1138 (2018).

    Article  Google Scholar 

  15. R. Atchudan, T. N. J. I. Edison, K. R. Aseer, S. Perumal, N. Karthik, and Y. R. Lee, Biosens. Bioelectron., 99, 303–311 (2018).

    Article  Google Scholar 

  16. R. Wang, K. Q. Lu, Z. R. Tang, and Y. J. Xu, J. Mater. Chem. A, 5, 3717–3734 (2017).

    Article  Google Scholar 

  17. A. Kumar, A. R. Chowdhuri, D. Laha, T. K. Mahto, P. Karmakar, and S. K. Sahu, Sensors Act. B: Chem., 242, 679–686 (2017).

    Article  Google Scholar 

  18. Y. Hou, H. Liu, Z. Li, H. Zhang, L. Wei, and M. Yu, Anal. Methods, 12, 2835–2840 (2020).

    Article  Google Scholar 

  19. F. Yuan, T. Yuan, L. Sui, Z. Wang, Z. Xi, Y. Li, X. Li, L. Fan, Z. Tan, and A. Chen, Nat. Commun., 9, Article ID 2249 (2018).

  20. F. Yuan, Z. Wang, X. Li, Y. Li, Z. Tan, L. Fan, and S. Yang, Adv. Mater., 29, Article ID 1604436 (2017).

  21. Y. Huan, Q. Fei, H. Shan, B. Wang, H. Xu, and G. Feng, Analyst., 140, 1655–1661 (2015).

    Article  ADS  Google Scholar 

  22. Y. Wu, Y. Gao, and J. Du, Talanta, 197, 599–604 (2019).

    Article  Google Scholar 

  23. Y. Nerthigan, A. K. Sharma, S. Pandey, K. H. Sharma, M. S. Khan, D. R. Hang, and H. F. Wu, Microchim. Acta, 185, 65 (2018).

    Article  Google Scholar 

  24. M. J. Cho and S. Y. Park, Sensors Act. B, 282, 719–729 (2019).

    Article  Google Scholar 

  25. C.-S. Chu, M.-W. Hsieh, and Z.-R. Su, Opt. Mater. Express, 6, 759–766 (2016).

    Article  ADS  Google Scholar 

  26. M. Tang, B. Zhu, Y. Wang, H. Wu, and Z. Su, Microchim. Acta, 186, 604–610 (2019).

    Article  Google Scholar 

  27. J. Wei, L. Qiang, J. Ren, X. Ren, F. Tang, and X. Meng, Anal. Methods, 6, 1922–1927 (2014).

    Article  Google Scholar 

  28. W. Hou, X. Liu, Q. Lu, M. Liu, Y. Zhang, and S. Yao, Colloids Surf. B: Biointerface, 162, 118–125 (2017).

    Article  Google Scholar 

  29. J. L. Ma, B. C. Yin, X. Wu, and B. C. Ye, Anal. Chem., 89, 1323–1328 (2017).

    Article  Google Scholar 

  30. H. Wang, Y. Xie, S. Liu, S. Cong, Y. Song, X. Xu, and M. Tan, J. Agric. Food Chem., 65, 7553–7559 (2017).

    Article  Google Scholar 

  31. X. Shan, L. Chai, J. Ma, Z. Qian, J. Chen, and F. Hui, Analyst., 139, 2322–2325 (2014).

    Article  ADS  Google Scholar 

  32. Y. Long, X. Wang, D. Shen, and H. Zheng, Talanta, 159, 122–126 (2016).

    Article  Google Scholar 

  33. L. Monti, S. Negri, A. Meucci, A. Stroppa, A. Galli, and G. Contarini, Food Chem., 220, 18–24 (2017).

    Article  Google Scholar 

  34. Y. H. Zhang, W. Zhao, Y. Y. Duan, D. P. Cao, J. Fang, and W. N. Chen, China Food Addit., 1, 137–140 (2016).

    Google Scholar 

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Authors and Affiliations

  1. School of Science at Jiangnan University, Wuxi, China

    Y. Wei, L. Li, Ch. Ma, Y. Wu, Ch. Zhu, H. Gao, J. Gu, Y. Xiong, X. Li, Z. Wang, G. Wang, S. Guo & G. Chen

  2. Jiangsu Provincial Research Center of Light Industrial Optoelectronic Engineering and Technology, Wuxi, China

    Y. Wei, L. Li, Ch. Ma, Y. Wu, Ch. Zhu, H. Gao, J. Gu, Y. Xiong, X. Li, Z. Wang, G. Wang, S. Guo & G. Chen

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  1. Y. Wei
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  2. L. Li
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  3. Ch. Ma
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  4. Y. Wu
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  5. Ch. Zhu
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  6. H. Gao
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  7. J. Gu
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  8. Y. Xiong
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  9. X. Li
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  10. Z. Wang
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  11. G. Wang
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  12. S. Guo
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  13. G. Chen
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Corresponding author

Correspondence to G. Chen.

Additional information

Published in Zhurnal Prikladnoi Spektroskopii, Vol. 89, No. 2, pp. 221–229, March–April, 2022.

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Wei, Y., Li, L., Ma, C. et al. Phenolic Hydroxyl Group–Carbon Dots as a Fluorescent Probe for the Detection of Hydrogen Peroxide and Glucose in Milk. J Appl Spectrosc 89, 272–280 (2022). https://doi.org/10.1007/s10812-022-01354-0

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  • DOI: https://doi.org/10.1007/s10812-022-01354-0

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