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Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)

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Abstract

RNA isolation is difficult in some plants and algae because phenolics, polysaccharides, or other compounds can bind or co-precipitate with RNA, and because the success of RNA isolation can be strain-specific and species-specific. To create an improved RNA isolation protocol for Laminaria japonica Aresch (Laminariaceae, Phaeophyta), four methods for extracting RNA were tested. A cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol was developed that clearly showed 28S and 18S ribosomal RNA bands and produced RNA with high yield (68 μg g−1 fresh weight) and high quality (A 260/280 ratio 1.96 ± 0.05). The isolated RNA was intact, and RT-PCR analysis confirmed that further molecular application is feasible.

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Acknowledgments

The authors gratefully acknowledge NSFC (40618001), the Shandong Agriculture Seed stock Breeding Project for financial assistance, and anonymous reviewers for their critical comments and suggestions to improve the manuscript. Thanks are also kindly given to Mr. Donald Sturge for English revision.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Jianting Yao, Wandong Fu, Xiuliang Wang & Delin Duan

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100059, China

    Jianting Yao & Wandong Fu

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  1. Jianting Yao
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  2. Wandong Fu
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  3. Xiuliang Wang
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  4. Delin Duan
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Correspondence to Delin Duan.

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Yao, J., Fu, W., Wang, X. et al. Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta). J Appl Phycol 21, 233–238 (2009). https://doi.org/10.1007/s10811-008-9354-0

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  • DOI: https://doi.org/10.1007/s10811-008-9354-0

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