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KCNQ1OT1 promotes retinoblastoma progression by targeting miR-339-3p that suppresses KIF23

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Abstract

Background

Long noncoding RNAs (lncRNAs) are involved in tumor formation and development. KCNQ1OT1 regulates the malignant proliferation of retinoblastoma (RB), but the specific mechanism remains to be further investigated.

Methods

The KCNQ1OT1, miR-339-3p and KIF23 expression levels in RB were detected by qRT-PCR and western blotting. The cell viability, proliferation, migration ability and caspase-3 activity of RB cells were evaluated by CCK-8, BrdU, transwell and caspase-3 activity analysis. Western blot was used to detect the Bax and Bcl-2 protein expression in RB cells. The binding relationship between KCNQ1OT1, miR-339-3p and KIF23 was detected by luciferase, RIP and RNA pull-down assay.

Results

KCNQ1OT1 and KIF23 were up-regulated frequently in RB, and miR-339-3p was down-regulated. Functional studies showed that downregulation of KCNQ1OT1 or KIF23 inhibited the survival and migration of RB cells, and facilitated apoptosis. Interference with miR-339-3p showed the opposite effect. Mechanisms suggested that KCNQ1OT1 exited its oncogenic activity by positively regulating the expression of KIF23 and sponging miR-339-3p.

Conclusion

KCNQ1OT1/miR-339-3p/KIF23 may be a new biomarker for the diagnosis and treatment of RB.

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Availability of data and material

The data used and analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

Not available.

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Authors and Affiliations

  1. Department of Ophthalmology, The First Affiliated Hospital of Chengdu Medical College, Chengdu, 610500, Sichuan, China

    Wenting Tang, Li Zhang & Jing Li

  2. Department of Ophthalmology, The 2nd Affiliated Hospital of Chengdu Medical College, Nuclear Industry 416 Hospital, No. 4, North 4th Erhuan Street, Chengdu, 610051, Sichuan, China

    Yu Guan

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Contributions

WT performed the experiments and data analysis. LZ and JL conceived and designed the study. YG made the acquisition of data. WT did the analysis and interpretation of data. All authors read and approved the manuscript.

Corresponding author

Correspondence to Yu Guan.

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The authors declare that they have no conflict of interests.

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The approval for the procedure was sanctioned by the Ethical Committee of The First Affiliated Hospital of Chengdu Medical College (Sichuan, China). The processing of clinical tissue samples is in strict compliance with the ethical standards of the Declaration of Helsinki. All the donors signed the written consent form.

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All patients signed written informed consent.

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Tang, W., Zhang, L., Li, J. et al. KCNQ1OT1 promotes retinoblastoma progression by targeting miR-339-3p that suppresses KIF23. Int Ophthalmol 43, 2419–2432 (2023). https://doi.org/10.1007/s10792-023-02641-1

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  • DOI: https://doi.org/10.1007/s10792-023-02641-1

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