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Involvement of protein kinase C in phagocytosis of human retinal pigment epithelial cells and induction of matrix metalloproteinase secretion

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Abstract

Protein kinase C (PKC) is involved in cell activation. We investigated PKC-mediated pathways and secretion of matrix metalloproteinases (MMPs) in phagocytosis by human retinal pigment epithelial cells (RPE). We used time-resolved fluorometry for europium-labeled microsphere uptake and gel zymography to assay the influence of PKC modulators. PKC inhibitors blocked phagocytosis by RPE. ARPE-19, a human RPE-cell line, showed reduced secretion of MMP-2, although MMP-9 secretion by PKC activation was conserved in both cell types, namely in the primary RPEs and in the RPE-cell line. Particle uptake by RPE cells requires activation of PKC; the use of PKC inhibitors as new anticancer drugs may possibly cause ocular side-effects.

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Abbreviations

MMP:

Matrix metalloproteinase

PKC:

Protein kinase C

PVR:

Proliferative vitreoretinopathy

RPE:

Retinal pigment epithelium

ARPE-19:

Human RPE cell line

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Acknowledgments

The authors thank Gabi Schweigl for technical assistance. This work is part of a thesis by Gertrud Haas. This work was supported by grants from “MFF Tirol” (Medical Science Fund Tyrol) and “Jubiläumsfonds” of the Austrian National Bank (Project No. 7749) to Eveline U. Irschick and Hartwig P. Huemer as well as from the FWF, SFB021 grant (“Cell proliferation and cell death in tumours”) to Florian Überall.

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Authors and Affiliations

  1. Clinical Department of Ophthalmology, Innsbruck Medical University, Anichstrasse 35, 6020, Innsbruck, Austria

    Eveline U. Irschick, Gertrud Haas & Josef Troger

  2. Department of Medical Chemistry and Biochemistry, Innsbruck Medical University, Pritz-Pregl-Strasse 3, 6020, Innsbruck, Austria

    Florian Ueberall

  3. Department of Hygiene, Microbiology and Social Medicine, Innsbruck Medical University, Fritz-Pregl-Strasse 3, 6020, Innsbruck, Austria

    Hartwig P. Huemer

Authors
  1. Eveline U. Irschick
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  2. Gertrud Haas
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  3. Josef Troger
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  4. Florian Ueberall
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  5. Hartwig P. Huemer
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Correspondence to Eveline U. Irschick.

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Irschick, E.U., Haas, G., Troger, J. et al. Involvement of protein kinase C in phagocytosis of human retinal pigment epithelial cells and induction of matrix metalloproteinase secretion. Int Ophthalmol 29, 333–341 (2009). https://doi.org/10.1007/s10792-008-9241-3

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  • DOI: https://doi.org/10.1007/s10792-008-9241-3

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