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The Relationship Between N-acetylcysteine, Hyperbaric Oxygen, and Inflammation in a Rat Model of Acetaminophen-induced Nephrotoxicity

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Abstract

An overdose of acetaminophen (APAP) produces acute tubular necrosis. The aim of this study was to observe the effects of hyperbaric oxygen (HBO) only and combined with N-acetylcysteine (NAC) on inflammatory cytokines in kidney. Thirty-two male Sprague–Dawley rats were divided into four groups: sham, control (APAP), NAC, and NAC + HBO. In the APAP, NAC, and NAC + HBO groups, renal injury was induced by oral administration of 1 g/kg APAP. The NAC group received NAC (100 mg/kg/day). NAC + HBO group received NAC (100 mg/kg/day) intraperitoneally and HBO underwent at 2.8 ATA pressure with 100 % oxygen inhalation for 90 min every 12 h for 5 days. Rats in the sham group received distilled water only by gastric tube. All animals were killed on 6 days after APAP or distilled water administration. Creatinine, urea, neopterin, tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 levels were measured in sera. There was a significant increase in serum creatinine and urea levels in the control group compared to the sham group (in both, p = 0.001). NAC and NAC + HBO significantly decreased serum neopterin, TNF-α, and IL-6 levels compared to control group. APAP administration caused tubular necrosis in the renal. NAC and NAC + HBO treatments significantly reduced APAP-induced renal damage. The results of this study showed that renal dysfunction in APAP toxicity was attenuated by the use of HBO and NAC treatments. The combination of NAC and HBO treatments might be recommended as an effective treatment modality for APAP-induced nephrotoxicity.

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The authors report no conflicts of interest.

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Authors and Affiliations

  1. Pathology Service, Etimesgut Military Hospital, 06790, Etimesgut, Ankara, Turkey

    Hakan Cermik

  2. Department of Biochemistry, Diskapi Yildirim Beyazit Training and Research Hospital, 06110, Diskapi, Ankara, Turkey

    Mine Yavuz Taslipinar & Fatma Ucar

  3. Department of Clinical Biochemistry, Gulhane Military Medical Academy, 06018, Etlik, Ankara, Turkey

    Ibrahim Aydin, Mehmet Agilli, Fevzi Nuri Aydin & Tuncer Cayci

  4. Department of Urology, Gulhane Military Medical Academy, 06018, Etlik, Ankara, Turkey

    Bilal Firat Alp

  5. Department of Forensic Medicine, Gulhane Military Medical Academy, 06018, Etlik, Ankara, Turkey

    Mehmet Toygar

  6. Department of Biochemistry, Ankara Occupational Diseases Hospital, 06280, Kecioren, Ankara, Turkey

    Esin Ozkan

  7. Department of Medical Biology, Gulhane Military Medical Academy, 06018, Etlik, Ankara, Turkey

    Ertan Altayli

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  1. Hakan Cermik
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Correspondence to Hakan Cermik.

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Cermik, H., Taslipinar, M.Y., Aydin, I. et al. The Relationship Between N-acetylcysteine, Hyperbaric Oxygen, and Inflammation in a Rat Model of Acetaminophen-induced Nephrotoxicity. Inflammation 36, 1145–1152 (2013). https://doi.org/10.1007/s10753-013-9649-4

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