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Early Increase in Alveolar Macrophage Prostaglandin 15d-PGJ2 Precedes Neutrophil Recruitment into Lungs of Cytokine-Insufflated Rats

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ABSTRACT

Early detection and prevention is an important goal in acute respiratory distress syndrome research. We determined the concentration of the anti-inflammatory 15-deoxy-Δ12,14-prostaglandin-J2 (15d-PGJ2) and other components of the cyclopentenone prostaglandin cascade in relation to lung inflammation in cytokine (IL-1/LPS)-insufflated rats. We found that 15d-PGJ2 levels increase in the bronchoalveolar lavage (BAL) fluid of rats insufflated with cytokines 2 h before. BAL 15d-PGJ2 increases preceded neutrophil recruitment, lung injury, and oxidative stress in the lungs of cytokine-insufflated rats. 15d-PGJ2 was localized in alveolar macrophages that decreased following cytokine insufflation. 15d-PGJ2 may constitute an early biomarker of lung inflammation and may reflect an endogenous attempt to regulate ongoing inflammation in macrophages and elsewhere after cytokine insufflation.

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Authors and Affiliations

  1. Department of Anesthesiology, University of Colorado SOM, AO-1, MS 8202, 12631 E 17th Ave, Aurora, CO, 80045, USA

    Ana Fernandez-Bustamante, Jelena Klawitter, Amanda Agazio & Uwe Christians

  2. Department of Medicine, University of Colorado SOM, Aurora, CO, USA

    John E. Repine

  3. Webb Waring Center, University of Colorado SOM, Aurora, CO, USA

    Ana Fernandez-Bustamante, Paul Wilson, Nancy D. Elkins, Amanda Agazio & John E. Repine

  4. iC42 Integrated Solutions in Systems Biology, University of Colorado SOM, Aurora, CO, USA

    Jelena Klawitter & Uwe Christians

  5. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan

    Takahiro Shibata & Koji Uchida

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  1. Ana Fernandez-Bustamante
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Correspondence to Ana Fernandez-Bustamante.

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Ana Fernandez-Bustamante and Jelena Klawitter contributed equally to this manuscript.

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Fernandez-Bustamante, A., Klawitter, J., Wilson, P. et al. Early Increase in Alveolar Macrophage Prostaglandin 15d-PGJ2 Precedes Neutrophil Recruitment into Lungs of Cytokine-Insufflated Rats. Inflammation 36, 1030–1040 (2013). https://doi.org/10.1007/s10753-013-9635-x

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