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Growth of HepG2 Cells was Suppressed Through Modulation of STAT6/IL-4 and IL-10 in RAW 264.7 Cells Treated by Phytoglycoprotein (38 kDa)

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Abstract

Macrophage type 2 (M2) is closely associated with tumor progression and metastasis. Thus, in this study, the antitumor effect of Styrax japonica Siebold et al. Zuccarini (SJSZ) glycoprotein on HepG2 cell proliferation through modulating M2 was investigated by measuring [3H]-thymidine incorporation and proliferating cell nuclear antigen (PCNA), nitric oxide (NO), reactive oxygen species (ROS), mitogen-activated protein kinases, signal transducer and activator of transcription (STAT) 6, cytokines [interleukin (IL)-4, IL-10, IL-12, and interferon (IFN)-γ], and CD163-positive cells using biochemical analysis, radioactivity, Western blot, ELISA, quantitative real-time polymerase chain reaction, and flow cytometry in coculture system. RAW 264.7 cells were found to be cytotoxic to HepG2 cells but [3H]-thymidine incorporation and expression of PCNA was suppressed in the presence of the SJSZ glycoprotein (20 μg/ml). The SJSZ glycoprotein normalized production of NO and ROS and expression of inducible nitric oxide synthase, IFN-γ, and IL-12 but suppressed expression of pSTAT6, IL-4, IL-10, and CD163-positive cells. Thus, the results of this study suggest that the SJSZ glycoprotein suppresses proliferation of HepG2 cells by modulating M2.

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Acknowledgments

This research was supported by the Priority Research Centers Program (2012-0005857) through the National Research Foundation from the Ministry of Education, Science and Technology, Korea Government.

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwangju, 500-757, South Korea

    Jin Lee

  2. Molecular Biochemistry Laboratory, Bioenergy Research Center, Chonnam National University, 300 Yongbong-Dong, Gwangju, 500-757, South Korea

    Kye-Taek Lim

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Correspondence to Kye-Taek Lim.

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Lee, J., Lim, KT. Growth of HepG2 Cells was Suppressed Through Modulation of STAT6/IL-4 and IL-10 in RAW 264.7 Cells Treated by Phytoglycoprotein (38 kDa). Inflammation 36, 549–560 (2013). https://doi.org/10.1007/s10753-012-9576-9

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  • DOI: https://doi.org/10.1007/s10753-012-9576-9

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