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Role of Interleukin-6 in Immune Complex Induced Models of Vascular Injury

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Abstract

Previous studies have suggested that Interleukin-6 (IL-6) acts as a marker of vasculitis. To determine the role of IL-6 in vasculitis we utilized two models of immune complex induced vascular injury (dermal Arthus and acute pulmonary alveolitis) in IL-6 deficient (IL-6−/−) and IL-6 sufficient (IL-6+/+) mice. Plasma and bronchoalveolar lavage (BAL) levels of IL-6 were elevated in the injured IL-6+/+ mice with acute alveolitis and in the plasma of IL-6+/+ mice with dermal Arthus vasculitis. While, IL-6 levels in IL-6−/− mice were near or below the levels of detection. Histological examination of the intensity of vascular injury response demonstrated no significant differences between IL-6−/− and IL6+/+ mice. More specifically, lung permeability (total protein in the BAL) in the lung injury model in IL-6−/− mice was the same as injured IL-6+/+ mice. As a corollary, assessment of vascular permeability in both models was the same in the IL-6−/− as the IL-6+/+ mice. Quantification of leukocyte influx into the injured tissues in both models also revealed no differences between the IL-6−/− and IL-6+/+ mice. These data demonstrate that while IL-6 is upregulated in acute vascular injury it does not appear to be critical in the development of the vascular inflammatory response.

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Authors and Affiliations

  1. Department of Pathology, University of Michigan Medical School, 7520 MSRB I, 1301 Catherine Rd., Ann Arbor, MI, 48109, USA

    Shannon D. McClintock, Adam G. Barron, Eric W. Olle, Michael P. Deogracias, Roscoe L. Warner & Kent J. Johnson

  2. Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA

    Mark Opp

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  1. Shannon D. McClintock
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  2. Adam G. Barron
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  3. Eric W. Olle
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  7. Kent J. Johnson
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Correspondence to Kent J. Johnson.

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This work is supported in part by: Pfizer Global Research & Development

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McClintock, S.D., Barron, A.G., Olle, E.W. et al. Role of Interleukin-6 in Immune Complex Induced Models of Vascular Injury. Inflammation 29, 154–162 (2005). https://doi.org/10.1007/s10753-006-9011-1

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