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From array to array: Confirmation of genomic gains and losses discovered by array-based comparative genomic hybridization utilizing fluorescence in situ hybridization on tissue microarrays

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Abstract

The combination of array-based comparative genomic hybridization (CGH) with fluorescence in situ hybridization utilizing custom-designed bacterial artificial chromosome (BAC) probes applied to tissue microarrays represents a powerful compendium of techniques–greatly enhancing the throughput of genomic analysis and subsequent target validation. Such approach can be automated at various levels and allows managing large volume of targets and samples in a few experiments. As such, this approach facilitates discovery, validation and implementation of findings in the process of identification of new diagnostic, prognostic and potentially therapeutic molecular markers.

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Authors and Affiliations

  1. Department of Anatomic Pathology, The Cleveland Clinic Foundation, 9500 Euclid Ave., L-25, Cleveland, OH, 44191, USA

    Marek Skacel & Ana Siva

  2. Clinical Pathology, The Cleveland Clinic Foundation, 9500 Euclid Ave., L-25, Cleveland, OH, 44191, USA

    Bo Xu & Raymond R Tubbs

Authors
  1. Marek Skacel
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  2. Ana Siva
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  3. Bo Xu
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  4. Raymond R Tubbs
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Correspondence to Marek Skacel.

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Skacel, M., Siva, A., Xu, B. et al. From array to array: Confirmation of genomic gains and losses discovered by array-based comparative genomic hybridization utilizing fluorescence in situ hybridization on tissue microarrays. J Mol Hist 38, 135–140 (2007). https://doi.org/10.1007/s10735-006-9051-8

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  • DOI: https://doi.org/10.1007/s10735-006-9051-8

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