Abstract
Geranyl diphosphate synthase (GPS) and farnesyl diphosphate synthase (FPS) catalyzes the biosynthesis of monoterpenoids and sesquiterpenoids, two key precursors for terpenoids biosynthesis. Here, the open reading frame (ORF) sequences of GPS (named CiGPS) and FPS (named CiFPS) with lengths of 1278 bp and 1035 bp were cloned from Chrysanthemum indicum var. aromaticum (C. indicum var. aromaticum). Phylogenetic analysis showed that CiGPS and CiFPS genes were closely related to Artemisia annua and Chrysanthemum × morifolium, respectively. qRT-PCR results showed that CiGPS and CiFPS gene had the highest expression level in leaves, while the expression of CiFPS was induced to methyl jasmonate. Overexpression of CiGPS and CiFPS in tobacco resulted in shorter plant height, darker leaf color, higher chlorophyll, and carotenoid content; the number of long handle trichomes increased significantly, while short handle trichomes only increased significantly on the upper epidermis of leaves of CiGPS transgenic tobacco lines. The relative content of terpenes of CiGPS and CiFPS transgenic tobacco lines increased significantly and monoterpenoids and sesquiterpenoids were detected in transgenic tobacco without wild type (WT) and empty vector (EV) lines. The activity of GPS enzyme was increased in CiGPS transgenic tobacco lines, but FPS enzyme activity in CiFPS transgenic tobacco lines was non-significant. Transcriptional analyses revealed that the expression levels of terpenoid biosynthesis pathway genes were higher in transgenic tobacco lines than in WT and EV lines. All the results demonstrated that GPS and FPS cDNA were effective to improve growth traits and alter terpenoid metabolism of C. indicum var. aromaticum.
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This research was supported by the National Natural Science Foundation of China (32001355), the Shanghai Sailing Program (20YF1447800). the Science and Technology Talent Development Fund for Young Teachers of Shanghai Institute of Technology (ZQ2020-4) and the Start-up Funding of Shanghai Institute of Technology (YJ2021-77).
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All authors contributed to the study conception and design. WG carried out the experiment, analyzed the data, and drafted the manuscript. QM and XW helped carried out the experiment and analyzed part of the data. FC reviewed and revised the manuscript. YZ and MH designed the experiments. All authors approved the final manuscript version.
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Gao, W., Meng, Q., Wang, X. et al. Overexpression of GPS and FPS from Chrysanthemum indicum var. aromaticum resulted in modified trichome formation and terpenoid biosynthesis in tobacco. Plant Growth Regul 99, 553–566 (2023). https://doi.org/10.1007/s10725-022-00927-5
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DOI: https://doi.org/10.1007/s10725-022-00927-5