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The calcium-dependent protein kinase gene VaCPK29 is involved in grapevine responses to heat and osmotic stresses

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Abstract

Ca2+-dependent protein kinases (CDPKs or CPKs) are essential primary sensors of Ca2+ in plants and are known to play important roles in plant abiotic and biotic stress responses. Vitis amurensis is a wild grapevine species with a high level of cold and disease resistance. It has previously been shown that transcription of 10 CDPK genes of V. amurensis was elevated under salt, desiccation, high mannitol, cold, and heat stress conditions. Expression of VaCPK29 was induced under high and low temperatures, water deficit, and high mannitol stress in plant cuttings of V. amurensis. The present study revealed that the callus cell cultures of V. amurensis and soil-grown plants of Arabidopsis thaliana overexpressing VaCPK29 exhibited higher tolerance to heat and high mannitol stress in comparison with the control transformed with the empty vector. Cold, salt, and drought stress tolerance of the transgenic V. amurensis calli and A. thaliana plants was comparable to that of the controls. The stress-responsive genes AtDREB1A, AtDREB2A, AtRD29A, AtRD29B, and AtABF3 were up-regulated in the VaCPK29-overexpressing A. thaliana plants under heat stress. Taken together, the data indicate that the VaCPK29 gene may act as a positive regulator in the grapevine response to heat and osmotic stresses.

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Acknowledgements

This work was supported by a Grant from the Russian Science Foundation (14-14-00366).

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Authors and Affiliations

  1. Laboratory of Biotechnology, Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia, 690022

    Alexandra S. Dubrovina, Konstantin V. Kiselev, Valeriya S. Khristenko & Olga A. Aleynova

  2. Department of Biochemistry, Microbiology and Biotechnology, Far Eastern Federal University, Vladivostok, Russia, 690090

    Konstantin V. Kiselev

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  1. Alexandra S. Dubrovina
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  2. Konstantin V. Kiselev
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  3. Valeriya S. Khristenko
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  4. Olga A. Aleynova
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Correspondence to Alexandra S. Dubrovina.

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Dubrovina, A.S., Kiselev, K.V., Khristenko, V.S. et al. The calcium-dependent protein kinase gene VaCPK29 is involved in grapevine responses to heat and osmotic stresses. Plant Growth Regul 82, 79–89 (2017). https://doi.org/10.1007/s10725-016-0240-5

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  • DOI: https://doi.org/10.1007/s10725-016-0240-5

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