Abstract
Using radioactive labeling technique it was analyzed proteins profiles of maize plants after treatment (12 h) with salt and brassinosteroids (BRs). Among separated proteins 66 and 67 proteins were observed specifically upregulated by salt treatment or BRs under salinity, respectively. Brassinazole—inhibitor of BRs biosynthesis—lowered BR-induced accumulation of proteins that confirm their BR-mediated synthesis. Data on 200 heat shock proteins (HSPs) from Arabidopsis thaliana and 42 HSPs from Zea mays have been used for polypeptide identification with bioinformatic approach. Protein bands separated on SDS-PAGE were compared with gene expression profile of HSPs from Genevestigator database in response to salinity and hormone treatment. In result, eight correlative matches were found between levels of polypeptide accumulation and activation of HSPs genes’ expression. It was suggested involvement of BRs in regulation of synthesis of mitochondrial chaperones under salinity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BRs:
-
Brassinosteroids
- BZR:
-
Brassinazole
- EBR:
-
24-epibrassinolide
- HSPs:
-
Heat shock proteins
- sHSPs:
-
Small heat shock proteins
References
Ali B, Hayat S, Fariduddin Q, Ahmad A (2008) 24-Epibrassinolide protects against the stress generated by salinity and nickel in Brassica juncea. Chemosphere 72:1387–1392
Banzet N, Richaud C, Deveaux Y, Kazmaier M, Gagnon J, Triantaphylidès C (1998) Accumulation of small heat shock proteins, including mitochondrial HSP22, induced by oxidative stress and adaptive response in tomato cells. Plant J 13:519–527. doi:10.1046/j.1365-313X.1998.00056.x
Bartwal A, Mall R, Lohani P, Guru S, Arora S (2013) Role of secondary metabolites and brassinosteroids in plant defense against environmental stresses. J Plant Growth Regul 32:216–232. doi:10.1007/s00344-012-9272-x
Bekh-Ochir D et al (2013) A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling. Planta 237:1509–1525. doi:10.1007/s00425-013-1859-3
Bressan RA, Zhang C, Zhang H, Hasegawa PM, Bohnert HJ, Zhu J-K (2001) Learning from the Arabidopsis experience. The next gene search paradigm. Plant Physiol 127:1354–1360. doi:10.1104/pp.010752
Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106
Derevyanchuk MV, Grabelnyh OI, Litvinovskaya RP, Voinikov VK, Sauchuk AL, Khripach VA, Kravets VS (2014) Influence of brassinosteroids on plant cell alternative respiration pathway and antioxidant systems activity under abiotic stress conditions. Biopolym Cell 30:436–442
Dhaubhadel S, Browning KS, Gallie DR, Krishna P (2002) Brassinosteroid functions to protect the translational machinery and heat-shock protein synthesis following thermal stress. Plant J 29:681–691
Downs CA, Heckathorn SA (1998) The mitochondrial small heat-shock protein protects NADH: ubiquinone oxidoreductase of the electron transport chain during heat stress in plants. FEBS Lett 430:246–250. doi:10.1016/S0014-5793(98)00669-3
Hu X, Li Y, Li C, Yang H, Wang W, Lu M (2010) Characterization of small heat shock proteins associated with maize tolerance to combined drought and heat stress. J Plant Growth Regul 29:455–464. doi:10.1007/s00344-010-9157-9
Imai J, Yahara I (2000) Role of HSP90 in salt stress tolerance via stabilization and regulation of calcineurin. Mol Cell Biol 20:9262–9270. doi:10.1128/mcb.20.24.9262-9270.2000
Inan G et al (2004) Salt cress. A halophyte and cryophyte Arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles. Plant Physiol 135:1718–1737. doi:10.1104/pp.104.041723
Kagale S, Divi U, Krochko J, Keller W, Krishna P (2007) Brassinosteroid confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses. Planta 225:353–364. doi:10.1007/s00425-006-0361-6
Kim DH, Xu ZY, Hwang I (2013) AtHSP17.8 overexpression in transgenic lettuce gives rise to dehydration and salt stress resistance phenotypes through modulation of ABA-mediated signaling. Plant Cell Rep 32:1953–1963. doi:10.1007/s00299-013-1506-2
Kim J, Ahn M, Park Y, Kim S, Min S, Jeong W, Liu J (2014) Synechocystis PCC6803 and PCC6906 dnaK2 expression confers salt and oxidative stress tolerance in Arabidopsis via reduction of hydrogen peroxide accumulation. Mol Biol Rep 41:1091–1101. doi:10.1007/s11033-013-2955-y
Ku MSB et al (1999) High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants. Nat Biotechnol 17:76–80
Kuzmin EV, Karpova OV, Elthon TE, Newton KJ (2004) Mitochondrial respiratory deficiencies signal up-regulation of genes for heat shock proteins. J Biol Chem 279:20672–20677. doi:10.1074/jbc.M400640200
Lee K-W, Cha J-Y, Kim K-H, Kim Y-G, Lee B-H, Lee S-H (2012) Overexpression of alfalfa mitochondrial HSP23 in prokaryotic and eukaryotic model systems confers enhanced tolerance to salinity and arsenic stress. Biotechnol Lett 34:167–174. doi:10.1007/s10529-011-0750-1
Lund AA, Blum PH, Bhattramakki D, Elthon TE (1998) Heat-stress response of maize mitochondria. Plant Physiol 116:1097–1110. doi:10.1104/pp.116.3.1097
Lutts S, Kinet JM, Bouharmont J (1996) Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance. Plant Growth Regul 19:207–218. doi:10.1007/bf00037793
Mostek A, Börner A, Badowiec A, Weidner S (2015) Alterations in root proteome of salt-sensitive and tolerant barley lines under salt stress conditions. J Plant Physiol 174:166–176. doi:10.1016/j.jplph.2014.08.020
Mu C, Zhang S, Yu G, Chen N, Li X, Liu H (2013) Overexpression of small heat shock protein LimHSP16.45 in Arabidopsis enhances tolerance to abiotic stresses. PLoS One 8:e82264. doi:10.1371/journal.pone.0082264
Nakashita H et al (2003) Brassinosteroid functions in a broad range of disease resistance in tobacco and rice. Plant J 33:887–898
Onda Y (2013) Oxidative protein-folding systems in plant cells. Int J Cell Biol 2013:15. doi:10.1155/2013/585431
Pegoraro C, Mertz L, Maia L, Rombaldi C, Oliveira A (2011) Importance of heat shock proteins in maize. J Crop Sci Biotechnol 14:85–95. doi:10.1007/s12892-010-0119-3
Samakovli D, Margaritopoulou T, Prassinos C, Milioni D, Hatzopoulos P (2014) Brassinosteroid nuclear signaling recruits HSP90 activity. New Phytol. doi:10.1111/nph.12843
Shigeta T et al (2014) Molecular evidence of the involvement of heat shock protein 90 in brassinosteroid signaling in Arabidopsis T87 cultured cells. Plant Cell Rep 33:499–510. doi:10.1007/s00299-013-1550-y
Siddique M, Gernhard S, Koskull-Döring P, Vierling E, Scharf K-D (2008) The plant sHSP superfamily: five new members in Arabidopsis thaliana with unexpected properties. Cell Stress Chaperones 13:183–197. doi:10.1007/s12192-008-0032-6
Smith CA, Melino VJ, Sweetman C, Soole KL (2009) Manipulation of alternative oxidase can influence salt tolerance in Arabidopsis thaliana. Physiol Plant 137:459–472. doi:10.1111/j.1399-3054.2009.01305.x
Song NH, Ahn YJ (2011) DcHsp17.7, a small heat shock protein in carrot, is tissue-specifically expressed under salt stress and confers tolerance to salinity. New Biotechnol 28:698–704. doi:10.1016/j.nbt.2011.04.002
Talaat NB, Shawky BT (2012) 24-Epibrassinolide ameliorates the saline stress and improves the productivity of wheat (Triticum aestivum L.). Environ Exp Bot 82:80–88. doi:10.1016/j.envexpbot.2012.03.009
Vera-Estrella R, Barkla BJ, Pantoja O (2014) Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics 111:113–127. doi:10.1016/j.jprot.2014.05.018
Xu J, Xue C, Xue D, Zhao J, Gai J, Guo N, Xing H (2013) Overexpression of GmHsp90s, a heat shock protein 90 (Hsp90) gene family cloning from soybean, decrease damage of abiotic stresses in Arabidopsis thaliana. PLoS One 8:e69810. doi:10.1371/journal.pone.0069810
Xue Y, Peng R, Xiong A, Li X, Zha D, Yao Q (2009) Yeast heat-shock protein gene HSP26 enhances freezing tolerance in Arabidopsis. J Plant Physiol 166:844–850. doi:10.1016/j.jplph.2008.11.013
Xue Y, Peng R, Xiong A, Li X, Zha D, Yao Q (2010) Over-expression of heat shock protein gene hsp26 in Arabidopsis thaliana enhances heat tolerance. Biol Plant 54:105–111. doi:10.1007/s10535-010-0015-1
Yu L et al (2010) Phosphatidic acid mediates salt stress response by regulation of MPK6 in Arabidopsis thaliana. New Phytol 188:762–773. doi:10.1111/j.1469-8137.2010.03422.x
Zhichang Z, Wanrong Z, Jinping Y, Jianjun Z, Xufeng LZL, Yang Y (2010) Over-expression of Arabidopsis DnaJ (Hsp40) contributes to NaCl-stress tolerance. Afr J Biotechnol 9:972–978
Zou J, Liu C, Liu A, Zou D, Chen X (2012) Overexpression of OsHsp17.0 and OsHsp23.7 enhances drought and salt tolerance in rice. J Plant Physiol 169:628–635. doi:10.1016/j.jplph.2011.12.014
Acknowledgments
We thank Dr. Jan Martinec for his critical reading and thoughtful comments on the manuscript. This work was supported by the State Fund for Fundamental Researches of Ukraine (Grants No. F54.4/026-2013), NAS of Ukraine Grant No. 2.1.10.32-10) and Belarusian Republican Foundation for Fundamental Research (Grant No. X13К-094).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that there are no conflicts of interest.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Derevyanchuk, M., Litvinovskaya, R., Khripach, V. et al. Brassinosteroid-induced de novo protein synthesis in Zea mays under salinity and bioinformatic approach for identification of heat shock proteins. Plant Growth Regul 78, 297–305 (2016). https://doi.org/10.1007/s10725-015-0093-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10725-015-0093-3