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In vitro polyploidization from shoot tips of Jatropha curcas L.: a biodiesel plant

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Abstract

Jatropha curcas L. has been considered one of the most promising alternatives for biofuel production and, thus, a relevant economic crop. In this context, in vitro tissue culture techniques such as organogenesis and embryogenesis have been conducted for mass clonal propagation of elite J. curcas lines. However, despite advancements, in vitro induction of polyploids has not yet been related for this crop. In this sense, the present study attempted to induce polyploidy in plantlets generated from shoot tips of J. curcas ‘Gonçalo’ (2n = 2× = 22 chromosomes, 2C = 0.85 pg). For this purpose, some criteria were adopted for selection of the most adequate colchicine treatment: (a) survival rate of the explants, and (b) number of tetraploid and (c) mixoploid plantlets. Tetraploid and mixoploid plantlets were obtained from different treatments, with 0.5 mM colchicine for 96 h being the most efficient. The plantlets were recovered and clonally propagated in tissue culture medium supplemented with indole-3-acetic acid and 6-benzylaminopurine. These results show that the tissue culture procedures were adequate for induction, propagation and recovery of tetraploid and mixoploid plantlets. Moreover, DNA ploidy level screening by flow cytometry was a practical and rapid strategy for selection of diploid, mixoploid and tetraploid plantlets. The tissue culture system presented here represents a reliable methodology for in vitro polyploid induction of this and other elite lines of J. curcas.

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Acknowledgments

The authors are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil), Fundação de Amparo à Pesquisa do Espírito Santo (FAPES, Vitória, ES, Brazil), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, MG, Brazil), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil) for financial support.

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Authors and Affiliations

  1. Laboratório de Citogenética, Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, ES, CEP: 29500-000, Brazil

    Stéfanie Cristina de Oliveira, Andrei Caíque Pires Nunes & Wellington Ronildo Clarindo

  2. Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Viçosa, MG, CEP: 36570-000, Brazil

    Carlos Roberto Carvalho

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  1. Stéfanie Cristina de Oliveira
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  2. Andrei Caíque Pires Nunes
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  3. Carlos Roberto Carvalho
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  4. Wellington Ronildo Clarindo
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Correspondence to Wellington Ronildo Clarindo.

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de Oliveira, S.C., Nunes, A.C.P., Carvalho, C.R. et al. In vitro polyploidization from shoot tips of Jatropha curcas L.: a biodiesel plant. Plant Growth Regul 69, 79–86 (2013). https://doi.org/10.1007/s10725-012-9749-4

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  • DOI: https://doi.org/10.1007/s10725-012-9749-4

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