Abstract
Water uptake across cell membranes is a principal requirement for plant growth at both the cellular and whole-plant levels. Water movement through plant membranes is regulated by aquaporins. We examined the expression of the OsAQP gene, encodes a tonoplast intrinsic protein (TIP), which was isolated from a rice panicle cDNA library. Semi-quantitative RT-PCR revealed that the gene was ubiquitously expressed in rice roots and leaves. Expression of the gene was up-regulated by drought, salinity and cold in leaves, down-regulated by these abiotic factors in roots, and the gene was also induced by the phytohormones gibberellic acid and abscisic acid in both leaves and roots. Expression of the gene was inhibited by salicylic acid, especially in roots. White light decreased levels of OsAQP transcript, whereas blue light increased expression of the gene. Given that the OsAQP gene is strongly expressed in response to drought, salinity, cold, abscisic acid and gibberellic acid, we propose that OsAQP is a stress-induced gene and that it plays an essential role in the defense of rice against several stresses.
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References
Agre P, Brown D, Nielsen S (1995) Aquaporin water channels: unanswered questions and unresolved controversies. Cur Opin Cell Biol 7(4):472–483
Alexandersson E, Fraysse L, Sjövall-Larsen S, Gustavsson S, Fellert M, Karlsson M, Johanson U, Kjellbom P (2005) Whole gene family expression and drought stress regulation of aquaporins. Plant Mol Biol 59(3):469–484
Basu D, Dehesh K, Schneider-Poetsch HJ, Harrington SE, McCouch SR, Quail PH (2000) Rice PHYC gene: structure, expression, map position and evolution. Plant Mol Biol 44(1):27–42
Bienert GP, Thorsen M, Schüssler MD, Nilsson HR, Wagner A, Tamás MJ, Jahn TP (2008) A subgroup of plant aquaporins facilitates the bi-directional diffusion of As (OH) 3 and Sb (OH) 3 across membranes. BMC Biol 10(6):26
Bienert GP, Bienert MD, Jahn TP, Boutry M, Chaumont F (2011) Solanaceae XIPs are plasma membrane aquaporins that facilitate the transport of many uncharged substrates. Plant J 66(2):306–317
Boursiac Y, Prak S, Boudet J, Postaire O, Luu DT, Tournaire-Roux C, Santoni V, Maurel C (2008) The response of Arabidopsis root water transport to a challenging environment implicates reactive oxygen species- and phosphorylation-dependent internalization of aquaporins. Plant Signal Behav 3(12):1096–1098
Cashmore AR, Jarillo JA, Wu YJ, Liu D (1999) Cryptochromes: blue light receptors for plants and animals. Science 284(5415):760–765
Chaumont F, Moshelion M, Daniels MJ (2005) Regulation of plant aquaporin activity. Biol Cell 97:749–764
Chinnusamy V, Schumaker K, Zhu JK (2004) Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants. J Exp Bot 55(395):225–236
Chinnusamy V, Gong Z, Zhu JK (2008) Abscisic acid-mediated epigenetic processes in plant development and stress responses. J Integr Plant Biol 50(10):1187–1195
Dehesh K, Tepperman J, Christensen AH, Quail PH (1991) phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol Gen Genet 225(2):305–313
Eisenbarth DA, Weig AR (2005) Dynamics of aquaporins and water relations during hypocotyls elongation in Ricinus communis seedlings. J Exp Bot 56(417):1831–1842
Forrest KL, Bhave M (2008) The PIP and TIP aquaporins in wheat form a large and diverse family with unique gene structures and functionally important features. Funct Integr Genomics 8(2):115–133
Fricke W, Peters WS (2002) The biophysics of leaf growth in salt-stressed barley. A study at the cell level. Plant Physiol 129(1):374–388
Hauser F, Horie T (2010) A conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K/Na ratio in leaves during salinity stress. Plant, Cell Environ 33:552–565
Huang GT, Ma SL, Bai LP, Zhang L, Ma H, Jia P, Liu J, Zhong M, Guo ZF (2012) Signal transduction during cold, salt, and drought stresses in plants. Mol Biol Rep 39(2):969–987
Hwang JU, Suh S, Yi H, Kim J, Lee Y (1997) Actin filaments modulate both stomatal opening and inward K+-channel activities in guard cells of Vicia faba L. Plant Physiol 115(2):335–342
Inoue S, Kinoshita T, Takemiya A, Doi M, Shimazaki K (2008) Leaf positioning of Arabidopsis in response to blue light. Mol Plant 1(1):15–26
Kay SA, Keith B, Shinozaki K, Chua NH (1989) The sequence of the rice phytochrome gene. Nucleic Acids Res 17(7):2865–2866
Li GW, Peng YH, Yu X, Zhang MH, Cai WM, Sun WN, Su WA (2008) Transport functions and expression analysis of vacuolar membrane aquaporins in response to various stresses in rice. J Plant Physiol 165(18):1879–1888
Liu Y-x, Liang W-h (2008) Bioinformatical analysis of OsAQP gene from rice. J Henan Normal Univ (Nat Sci) 36(2):117–119 (in Chinese)
Liu Y-x, Liang W-h, Zhang L-j (2008) Expression analysis on OsAQP gene in rice leaves. Chinese J Rice Sci 22(5):545–547 (in Chinese)
Liu Q, Umeda M, Uchimiya H (1994) Isolation and expression analysis of two rice genes encoding the major intrinsic protein. Plant Mol Biol 26:2003–2007
Lu P, Zhang SQ, Outlaw WH Jr, Riddle KA (1995) Sucrose: a solute that accumulates in the guard cell apoplast and guard cell symplast of open stomata. FEBS Lett 362(2):180–184
Neff MM, Fankhauser C, Chory J (2000) Light: an indicator of time and place. Genes Dev 14(3):257–271
Netting AG (2000) PH. Abscisic acid and the integration of metabolism in plants under stressed and non-stressed conditions: cellular responses to stress and their implication for plant water relations. J Exp Bot 51(343):147–158
Ozga JA, van Huizen R, Reinecke DM (2002) Hormone and seed-specific regulation of pea fruit growth. Plant Physiol 128(4):1379–1389
Peng YH, Lin WL, Cai WM, Arora R (2007) Overexpression of a Panax ginseng tonoplast aquaporin alters salt tolerance, drought tolerance and cold acclimation ability in transgenic Arabidopsis plants. Planta 226:729–740
Quail PH (2002a) Photosensory perception and signalling in plant cells: new paradigms? Curr Opin Cell Biol 14(2):180–188
Quail PH (2002b) Phytochrome photosensory signalling networks. Nat Rev Mol Cell Biol 3(2):85–93
Ritte G, Rosenfeld J, Rohrig K, Raschke K (1999) Rates of sugar uptake by guard cell protoplasts of Pisum sativun L. related to the solute requirement for stomatal opening. Plant Phsyiol 121(2):647–655
Rivas-San Vicente M, Plasencia J (2011) Salicylic acid beyond defence: its role in plant growth and development. J Exp Bot 62(10):3321–3338
Sade N, Vinocur BJ, Diber A, Shatil A, Ronen G, Nissan H, Wallach R, Karchi H, Moshelion M (2009) Improving plant stress tolerance and yield production: is the tonoplast aquaporin SlTIP2; 2 a key to isohydric to anisohydric conversion? New Phytol 181:651–661
Sakurai J, Ishikawa F, Yamaguchi T, Uemura M, Maeshima M (2005) Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiol 46(9):1568–1577
Shan X, Yan J, Xie D (2012) Comparison of phytohormone signaling mechanisms. Curr Opin Plant Biol 15(1):84–91
Sharkey TD, Raschke K (1981) Effect of light quality on stomatal opening in leaves of Xanthium strumarium L. Plant Physiol 68(5):1170–1174
Takahashi H, Rai M, Kitagawa T, Morita S, Masumura T, Tanaka K (2004) Differential localization of tonoplast intrinsic proteins on the membrane of protein body type II and aleurone grain in rice seeds. Biosci Biotechnol Biochem 68(8):1728–1736
Takano M, Kanegae H, Shinomura T, Miyao A, Hirochika H, Furuya M (2001) Isolation and characterization of rice phytochrome A mutants. Plant Cell 13(3):521–534
Takano M, Inagaki N, Xie X, Yuzurihara N, Hihara F, Ishizuka T, Yano M, Nishimura M, Miyao A, Hirochika H, Shinomura T (2005) Distinct and cooperative functions of phytochromes A, B, and C in the control of deetiolation and flowering in rice. Plant Cell 17(12):3311–3325
Takase T, Ishikawa H, Murakami H, Kikuchi J, Sato-Nara K, Suzuki H (2011) The circadian clock modulates water dynamics and aquaporin expression in Arabidopsis roots. Plant Cell Physiol 52(2):373–383
Talbott LD, Zeiger E (1996) Central roles for potassium and sucrose in guard cell osmoregulation. Plant Physiol 111(4):1051–1057
Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K (2000) Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci USA 97(21):11632–11637
Voicu MC, Cooke JE, Zwiazek JJ (2009) Aquaporin gene expression and apoplastic water flow in bur oak (Quercus macrocarpa) leaves in relation to the light response of leaf hydraulic conductance. J Exp Bot 60(14):4063–4675
Wan XR, Li L (2006) Regulation of ABA level and water-stress tolerance of Arabidopsis by ectopic expression of a peanut 9-cis-epoxycarotenoid dioxygenase gene. Biochem Biophys Res Commun 347(4):1030–1038
Wang H, Deng XW (2003) Dissecting the phytochrome A-dependent signaling network in higher plants. Trends Plant Sci 8(4):172–178
Wang X, Li Y, Ji W, Bai X, Cai H, Zhu D, Sun XL, Chen LJ, Zhu YM (2011a) A novel Glycine soja tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic Arabidopsis thaliana. J Plant Physiol 168(11):1241–1248
Wang ZY, Xiong L, Li W, Zhu JK, Zhu J (2011b) The plant cuticle is required for osmotic stress regulation of abscisic acid biosynthesis and osmotic stress tolerance in Arabidopsis. Plant Cell 23(5):1971–1984
Yang H-M, Wang G-X (2001) The relationships between the variations of cytosolic Ca2+ concentration in guard cells and the stomatal movements. Plant Physiol Commun 37:269–273
Zhu C, Schraut D, Hartung W, Schaffner AR (2005) Differential responses of maize MIP genes to salt stress and ABA. J Exp Bot 56:2971–2981
Acknowledgments
We thank anonymous reviewers for helpful editorial comments. This work are supported by National Science Foundation of China (No. 31171182), Key Project of Chinese Ministry of Education (No.209076), Innovation Scientists and Technicians Troop Construction Projects of Henan Province (No. 104100510012) and Henan Natural Science Research project (No. 2010A180012).
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Liang, Wh., Li, L., Zhang, F. et al. Effects of abiotic stress, light, phytochromes and phytohormones on the expression of OsAQP, a rice aquaporin gene. Plant Growth Regul 69, 21–27 (2013). https://doi.org/10.1007/s10725-012-9743-x
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DOI: https://doi.org/10.1007/s10725-012-9743-x