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Quantitative dissection of lipid degradation in rice seeds during accelerated aging

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Abstract

Aging during storage negatively affects rice seed viability and nutrition quality. Lipid degradation caused by phospholipase D (PLD) activity is known to be responsible for seed deterioration in Arabidopsis, but the mechanisms of this process in monocotyledonous plant rice remain unclear. In this study, we carried out lipid profiling analysis for rice seeds, and found that the main membrane lipids phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) declined during accelerated aging. In contrast, phosphatidic acid (PA), a hydrolysis product produced by PLD, increased. Interestingly, the degree of PA increase induced by accelerated aging (at 2 days) was much higher in rice than that in Arabidopsis (200% vs. 30%). Moreover, the decreased molecular species in PC and PE were well fit with those that were increased in PA, suggesting that PA may be derived from PLD-mediated lipid hydrolysis. OsPLDα1 and α3 were the major PLDαs in rice seeds, and their transcription increased significantly during the aging process. The aging treatment induced OsPLDα protein expression and activation. Three lipoxygenases (LOXs) were analyzed, and OsLOX2 transcription was induced significantly during seed aging. Our results reveal detailed information regarding lipid degradation during rice seed aging, and we go on to discuss the relationship between lipid degradation and peroxidation during seed aging.

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Abbreviations

AA:

Accelerated aging

DGDG:

Digalactosyldiacylglycerol

ESI–MS/MS:

Electrospray ionization tandem mass spectrometry

H2O2 :

Hydrogen peroxide

JA:

Jasmonic acid

LOX:

Lipoxygenase

LysoPC:

Lysophosphatidylcholine

LysoPG:

Lysophosphatidylglycerol

LysoPE:

Lysophosphatidylethanolamine

MDA:

Malondialdehyde

MGDG:

Monogalactosyldiacylglycerol

PA:

Phosphatidic acid

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PI:

Phosphatidylinositol

PLA:

Phopholipase A

PLD:

Phospholipase D

PS:

Phosphatidylserine

PUFA:

Polyunsaturated fatty acid

qRT-PCR:

Quantitative reverse transcription-polymerase chain reaction

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Acknowledgments

This work was supported by grants from the National Science Foundation of China (30625027), the Ministry of Science and Technology of China (2006CB100100, 2008AA10Z122), the 111 project (B07030), the Fundamental Research Funds for the Central Universities (KYT201001), and from the Education Department of Jiangsu (200910, PAPD) to Wenhua Zhang. We thank Ruth Welti and Mary Roth at the Kansas Lipidomics Research Center for lipid profiling.

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Authors and Affiliations

  1. College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Fawei Wang, Rong Wang, Wen Jing & Wenhua Zhang

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  1. Fawei Wang
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  2. Rong Wang
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  3. Wen Jing
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  4. Wenhua Zhang
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Correspondence to Wenhua Zhang.

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Wang, F., Wang, R., Jing, W. et al. Quantitative dissection of lipid degradation in rice seeds during accelerated aging. Plant Growth Regul 66, 49–58 (2012). https://doi.org/10.1007/s10725-011-9628-4

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