Skip to main content
SpringerLink
Log in

An alginate-derived oligosaccharide enhanced wheat tolerance to cadmium stress

  • Original Research
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Hydroponic experiments were carried out to study the role of alginate-derived oligosaccharides (ADO) in enhancing wheat (Triticum aestivum L.) tolerance to cadmium stress. Data were collected on plant biomass, chlorophyll content, photosynthetic rate, antioxidant enzyme activity and malondialdehyde (MDA) content. Under 100 μM Cd stress, plant growth was significantly inhibited. Shoot length, root length, fresh and dry weight were sharply reduced by 24.21, 34.59, 22.1 and 14.7%, respectively of the control after 10 day of Cd exposure. Superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities were increased and MDA content increased. Wheat seeds were soaked for 5 h in 1,000 mg L−1 ADO solution before cadmium stress. ADO pretreatment alleviated cadmium toxicity symptoms, which were reflected by increasing root and shoot lengths, fresh and dry weight, chlorophyll content and photosynthetic rate (P n ). Furthermore, ADO pretreatment significantly increased antioxidant enzyme (SOD, CAT and POD) activities and reduced MDA content in leaves and roots. The results indicated that ADO pretreatment partially protected the seedlings from cadmium toxicity during the following growth period.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Akimoto C, Aoyagi H, Tanaka H (1999) Endogenous elicitor-like effects of alginate on physiological activities of plant cells. Appl Microbiol Biotechnol 52:429–436

    Article  CAS  Google Scholar 

  • Albersheim P, Darvill AG (1985) Oligosaccharins: novel molecules that can regulate growth, development, reproduction, and defense against disease in plants. Sci Am 253:58–64

    Article  Google Scholar 

  • Alvarez M, Pennell R, Meijer P, Ishikawa A, Dixon R, Lamb C (1998) Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92:773–784

    Article  CAS  PubMed  Google Scholar 

  • Anjum NA, Umar S, Ahmad A, Iqbal M, Khan NA (2008) Sulphur protects mustard (Brassica campestris L.) from cadmium toxicity by improving leaf ascorbate and glutathione. Plant Growth Regul 54:271–279

    Article  CAS  Google Scholar 

  • Cakmak I, Horst WJ (1991) Effect of aluminum on lipid peroxidation, superioxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant 83:463–468

    Article  CAS  Google Scholar 

  • Chaoui A, Mazhoudi S, Ghorbal MH, Ferjani EL (1997) Cadmium and zinc induction of lipid peroxideation and effects on antioxidant enzyme activities in bean (Phaseolus ulgaris L.). Plant Sci 127:139–147

    Article  CAS  Google Scholar 

  • Cheng W, Zhang G, Yao H, Zhang H (2008) Genotypic difference of germination and early seedling growth response to Cd stress and its relation to Cd accumulation. J Plant Nutr 11:702–715

    Article  Google Scholar 

  • Costa H, Gallego SM, Tomaro ML (2002) Effects of UV-B radiation on antioxidant defense system in sunflower cotyledons. Plant Sci 162:939–945

    Article  CAS  Google Scholar 

  • DalCorso G, Farinati S, Maistri S, Furini A (2008) How plants cope with cadmium: staking all on metabolism and gene expression. J Integr Plant Biol 50:1268–1280

    Article  CAS  PubMed  Google Scholar 

  • Edreva A (2005) Generation and scavenging of reactive oxygen species in chloroplasts: a submolecular approach. Agric Ecosyst Environ 106:119–133

    Article  CAS  Google Scholar 

  • Guo B, Liang YC, Li ZJ, Guo W (2007) Role of salicylic acid in alleviating cadmium toxicity in rice roots. J Plant Nutr 30:427–439

    Article  CAS  Google Scholar 

  • Hassan MJ, Shfi M, Zhang GP, Zhu ZJ, Qaisar M (2008) The growth and some physiological responses of rice to Cd toxicity as affected by nitrogen form. Plant Growth Regul 54:125–132

    Article  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  CAS  PubMed  Google Scholar 

  • Hsu YT, Kao CH (2007) Toxicity in leaves of rice exposed to cadmium is due to hydrogen peroxide accumulation. Plant Soil 298:231–241

    Article  CAS  Google Scholar 

  • Hu XK, Jiang XL, Hueymin H, Liu SL, Guan HS (2004) Promotive effects of alginate-derived oligosaccharide on maize seed germination. Appl Phys 16:73–76

    CAS  Google Scholar 

  • Hu YL, Ge Y, Zhang CH, Ju T, Cheng WD (2009) Cadmium toxicity and translocation in rice seedling are reduced by hydrogen peroxide pretreatment. Plant Growth Regul 58:47–59

    Article  Google Scholar 

  • Kumar P, Tewari PK, Sharma PN (2008) Cadmium enhances generation of hydrogen peroxide and amplifies activities of catalase, peroxidases and superoxide dismutase in maize. J Agron Crop Sci 194:72–80

    Article  CAS  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  • Meng HB, Hua SJ, Shamsi IH, Jilani G, Li YL, Jiang LX (2009) Cadmium- induced stress on the seed germination and seedling growth of Brassica napus L., and its alleviation through exogenous plant growth regulators. Plant Growth Regul 58:47–59

    Article  CAS  Google Scholar 

  • Metwally A, Finkemeier I, Georgi M, Dietz KJ (2003) Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiol 132:272–281

    Article  CAS  PubMed  Google Scholar 

  • Milone MT, Sgherri C, Clijsters H, Navari-Izzo F (2003) Antioxidative responses of wheat treated with realistic concentrateion of cadmium. Environ Exp Bot 50:265–276

    Article  CAS  Google Scholar 

  • Molina AS, Nievas C, Chaca MVP, Garibotto F, Gonza’lez U, Marsá SM, Luna C, Gime’nez MS, Zirulnik F (2008) Cadmium- induced oxidative damage and antioxidantive defense mechanisms in Vigna mungo L. Plant Growth Regul 56:285–295

    Article  CAS  Google Scholar 

  • Natsume M, Kamo Y, Hirayama M, Adachi T (1994) Isolation and characterization of alginate-derived oligosaccharides with root growth-promoting activities. Carbohydr Res 258:187–197

    Article  CAS  PubMed  Google Scholar 

  • Penner GA, Clarke J, Bezte LJ, Leisle D (1995) Identification of RAPD markers linked to a gene governing cadmium uptake in durum wheat. Genome 38:543–547

    CAS  PubMed  Google Scholar 

  • Romero-Puertas MC, Rodriguez-Serrano M, Corpas FJ, Gómez M, del Rio LA, Sandalio LM (2004) Cadmium-induced subcellular accumulation of O2 − and H2O2 in pea leaves. Plant Cell Environ 27:1122–1134

    Article  CAS  Google Scholar 

  • Sandalio LM, Dalruzo HC, Gomez M, Romero-Puetras MC, del-Rio LA (2001) Cadmium- induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot 52(364):2115–2126

    CAS  PubMed  Google Scholar 

  • Shah K, Kumar RG, Verma S, Dubey RS (2001) Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Sci 161:1135–1144

    Article  CAS  Google Scholar 

  • Shamsi IH, Jilani G, Zhang GP, Kang W (2008) Interactive effects of cadmium and aluminum on growth and antioxidative enzymes in soybean. Biol Plant 52:165–169

    Article  CAS  Google Scholar 

  • Tomoda Y, Umemura K, Adachi T (1994) Promotion of barley root elongateion under hypoxic conditions by alginate lyase-lysate. Biosci Biotechnol Biochem 58:202–203

    Article  CAS  Google Scholar 

  • Weckx JEJ, Clijsters HMM (1997) Zinc phytotoxicity induces oxidative stress in primary leaves of Phaseolus vulgaris. Plant Physiol Biochem 35:405–410

    CAS  Google Scholar 

  • Wu FB, Zhang GP (2004) Effect of cadmium on free amino acids, glutathione and ascorbic acid content in two barley genotypes differing in Cd tolerance. Chemosphere 57:447–454

    Article  CAS  PubMed  Google Scholar 

  • Yin H, Chen Q, Yi M (2008) Effects of short-term heat stress on oxidative damage and responses of antioxidant system in lilium longiflorum. Plant Growth Regul 54:45–54

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (30870205), the Department of Education of Liaoning province Foundation (L2010516) and the Liaoning province Natural Science Foundation (20052053) and Director Foundation of Experimental Center, Shenyang Normal University (sy200705).

Author information

Authors and Affiliations

  1. College of Chemistry and Life Science, Shenyang Normal University, 253, huanghe Street, 110034, Shenyang, China

    L. J. Ma, X. M. Li, N. Bu & N. Li

Authors
  1. L. J. Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. X. M. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. Bu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. Bu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ma, L.J., Li, X.M., Bu, N. et al. An alginate-derived oligosaccharide enhanced wheat tolerance to cadmium stress. Plant Growth Regul 62, 71–76 (2010). https://doi.org/10.1007/s10725-010-9489-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-010-9489-2

Keywords

Search

Navigation