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Phospholipid and phospholipase changes by jasmonic acid during stolon to tuber transition of potato

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Abstract

Potato tuber formation starts with the stolon swelling and is regulated by jasmonates. The cascade of events leading to tuber formation is not completely understood. The aim of this study was to evaluate phospholipid composition and phospholipase activities during four stages of stolon-to-tuber transition of Solanum tuberosum L., cv. Spunta, and involvement of phosphatidic acid (PA) in stolon cell expansion during early stages. Effects of jasmonic acid (JA) treatment on phospholipid content and activation of phospholipase D (PLD) (EC 3.1.4.4) and phosphatidylinositol-4,5-bisphosphate-specific phospholipase C (PIP2-PLC) (EC 3.1.4.3) were studied in the early stages (first stage, hooked apex stolon; second stage, initial swelling stolon) of tuberization. All the phospholipid species identified, phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), PA, and cardiolipin (CL), decreased as tuber formation progressed. PLD and PLC were activated in control tissues at an early stage. JA treatment caused a decrease of PC and PS in first stage stolons, accumulation of PA in second stage stolons, and modification of PLD and PLC activities. PA increased stolon cell area in the first and second stages. These findings indicate that phospholipid catabolism is activated from the early stages of tuber formation, and that JA treatment modifies the pattern of phospholipid (PC, PS, and PA) composition and phospholipase (PLD and PLC) activity. These phospholipids therefore may play a role in activation of an intracellular mechanism that switches the developmental fate of stolon meristem cells, causing differentiation into a tuber.

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Abbreviations

CL:

Cardiolipin

JA:

Jasmonic acid

PA:

Phosphatidic acid

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PI:

Phosphatidylinositol

PIP2 :

Phosphatidylinositol-4,5-bisphosphate

PIP2-PLC:

Phosphatidylinositol-4,5-bisphosphate specific phospholipase C

PS:

Phosphatidylserine

PLD:

Phospholipase D

All solvent proportions are by volume

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Acknowledgements

This work was supported by grants from SECYT-UNRC and CONICET to G.A., and fellowships from CONICET to A.C.

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Authors and Affiliations

  1. Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, CP 5800, Rio Cuarto, Cordoba, Argentina

    Ana Cenzano, Renata Cantoro & Guillermina Abdala

  2. Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800, Rio Cuarto, Argentina

    Graciela Racagni

  3. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A. C. (CICY), Calle 43 # 130 Col. Chuburná de Hidalgo, CP 97200, Merida, Yucatan, Mexico

    Cesar De Los Santos-Briones & Teresa Hernández-Sotomayor

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  1. Ana Cenzano
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  2. Renata Cantoro
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  3. Graciela Racagni
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  4. Cesar De Los Santos-Briones
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  5. Teresa Hernández-Sotomayor
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  6. Guillermina Abdala
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Correspondence to Ana Cenzano.

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Cenzano, A., Cantoro, R., Racagni, G. et al. Phospholipid and phospholipase changes by jasmonic acid during stolon to tuber transition of potato. Plant Growth Regul 56, 307–316 (2008). https://doi.org/10.1007/s10725-008-9311-6

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