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Repetitive somatic embryogenesis in carnation on picloram supplemented media

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Abstract

An efficient method of repetitive somatic embryogenesis and plant regeneration of two carnation cultivars (Sagres and Impulse) was established using a two-step protocol. In the first step, embryogenic tissue were induced from petal explants on MS culture medium containing 9% sucrose (w/v), 9 μM 2,4-D and 0.8 μM BA. In the second step, embryogenic tissue transferred onto the MS medium containing 3% sucrose supplemented with different concentrations of picloram (0.8, 2, 4, 8 and 16 μM) to produce primary somatic embryos. Precotyledonary clumps and cotyledonary somatic embryos were then isolated and subcultured onto the same media as the second step where they formed secondary somatic embryos in repetitive cycles. Cotyledony somatic embryos were converted into plantlets when they transferred onto the growth regulator-free half-strength MS medium followed by being acclimated to the greenhouse conditions.

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Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

MS:

Murashige and Skoog (1962) basal medium

Picloram:

4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid

NAA:

α-naphthalene acetic acid

IBA:

Indole-3-butyric acid

IAA:

Indole acetic acid

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran

    Omid Karami & Ali Deljou

  2. Department of Horticulture, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran

    Aghil Mahmodi Pour

Authors
  1. Omid Karami
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  2. Ali Deljou
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  3. Aghil Mahmodi Pour
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Correspondence to Omid Karami.

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Karami, O., Deljou, A. & Pour, A.M. Repetitive somatic embryogenesis in carnation on picloram supplemented media. Plant Growth Regul 51, 33–39 (2007). https://doi.org/10.1007/s10725-006-9144-0

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  • DOI: https://doi.org/10.1007/s10725-006-9144-0

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