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Assessment of genetic diversity of Prunus salicina 'Shazikongxinli' by morphological traits and molecular markers

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Abstract

Prunus salicina 'Shazikongxinli' is one of China’s most economically valuable and reputable Prunus salicina cultivars. Understanding the genetic diversity and population structure of 'Shazikongxinli' is imperative for excellent germplasm breeding and the preservation of genetic resources. This work used morphological markers, inter-retrotransposon amplified polymorphism (IRAP), and inter simple sequence repeat (ISSR) to assess the genetic variation status of 50 'Shazikongxinli', with 18 plum cultivars selected as the control outgroup. The results showed that the average Shannon–Weaver diversity index (H') of 32 qualitative traits in 50 tests 'Shazikongxinli' was 0.557, the average coefficient of variation of 11 quantitative traits was 15.57%, and a total of 232 and 105 polymorphic loci were obtained from 22 IRAP and 15 ISSR primers, respectively. All three marker systems showed relatively rich polymorphism, especially the IRAP markers. This might be related to the nature of the retrotransposons in the IRAP markers, which might be more suitable for intraspecific variability detection than ISSR. In addition, all three markers clustered the 68 tested germplasms into two groups. All of 'Shazikongxinli' clustered into one group, and most outgroup plum cultivars clustered into another group. This suggested a relatively narrow genetic base within the 'Shazikongxinli' population. These results would be useful in understanding the genetic diversity of the germplasm resources of 'Shazikongxinli' and provide a basic theoretical reference for breeding superior germplasm.

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Funding

The project was supported by grants from the Science and Technology Program of Guizhou Province, China (Grant Numbers [2019] 4013 and [2020] 1Y023).

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Authors and Affiliations

  1. Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountain Region, (Ministry of Education), Institute of School of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, China

    Gui Wang, Ruirui Li & Guang Qiao

  2. Tongren Academy of Forestry, Tongren, 554300, Guizhou, China

    Maohong Wu

  3. Tongren Academy of Agricultural Sciences, Tongren, 554300, Guizhou, China

    Feihong Ren

  4. Guizhou Academy of Agricultural Sciences, Guiyang, 550006, Guizhou, China

    Lili Wang

Authors
  1. Gui Wang
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  2. Ruirui Li
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  3. Maohong Wu
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  4. Feihong Ren
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  5. Lili Wang
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  6. Guang Qiao
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Contributions

QG conceived and designed the experiments. WG and LRR performed the experiments. WG and WLL analyzed the data and wrote the paper. RFH collected samples and managed the materials. WMH obtained the funding. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Guang Qiao.

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The authors declare that there are no conflicts of interest. All the authors read and approved the manuscript in its final form.

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The datasets generated and analyzed during the current study are available from the corresponding author upon reasonable request.

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Wang, G., Li, R., Wu, M. et al. Assessment of genetic diversity of Prunus salicina 'Shazikongxinli' by morphological traits and molecular markers. Genet Resour Crop Evol 70, 2727–2739 (2023). https://doi.org/10.1007/s10722-023-01599-4

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