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Contribution to the knowledge of the genetic diversity and taxonomy of some Iranian Trifolium species

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Abstract

Trifolium (Fabaceae) is widely distributed with more than 255 species. The current study contributed to the knowledge of the diversity and genetic population structure of eight Iranian Trifolium, which remained relatively obscure. The taxonomic circumscription of sections and varieties is still questionable and poorly understood. The current study contributed to the genetic diversity, and population genetics of eight Iranian Trifolium specimens belonging to sections Mistyllus and Vesicaria based on IRAP and SSR datasets. A considerable level of polymorphism was detected among specimens. Molecular variance analysis exhibited much more genetic variation within populations in IRAP genetic marker, while an equal level of variation was detected within and among populations in SSR. IRAP and SSR genetic markers exhibited significant tree incongruence mainly due to the existence of a different genomic constitution. Furthermore, the results confirmed that the Vesicaria and Mistyllus sections are completely separate.

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Data availability

All analyzed data are available as Online Resources associated with this manuscript and the voucher specimens have deposited in the Herbarium of the University of Isfahan (HUI).

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Acknowledgements

The authors would like to thank Dr. Mohammad Reza Rahiminejad and to appreciate Payame Noor and Isfahan Universities for their financial support.

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No funding was received.

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Contributions

MH carried out the fieldwork as well as lab studies. AEF wrote the first draft of the manuscript, which edited by MH. Analyses were also checked by both authors.

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Correspondence to Maryam Haerinasab.

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The authors have carried out fieldwork and data generation ethically, including obtaining appropriate permitting.

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Haerinasab, M., Eslami-Farouji, A. Contribution to the knowledge of the genetic diversity and taxonomy of some Iranian Trifolium species. Genet Resour Crop Evol 69, 699–717 (2022). https://doi.org/10.1007/s10722-021-01254-w

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  • DOI: https://doi.org/10.1007/s10722-021-01254-w

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