Abstract
The study deals with similarity among 25 species of the genus Trifolium represented by 134 accessions. Clustering of the species based on isozyme banding pattern of five enzymes revealed that T. repens was distinctly different from other species. T. repens and T. retusum formed independent clusters. The group of species comprising of T. pratense, T. cherleri, T. spumosum, T. subterraneum, T. resupinatum, T. alexandrinum, T. echinatum, T. constantinopolitanum and T. tembense exhibited considerable similarity to the second cluster. This group joined another group of five species, i.e. T. nigrescens, T. glomeratum, T. apertum, T. alpestre and T. hybridum with nearly 50% similarity. T. purpureum, T. hirtum, T. campestre, T. incarnatum, and T. argutum grouped separately. There was no marked difference for banding pattern among T. alexandrinum genotypes. T. alexandrinum showed close affinity with T. subterraneum and T. resupinatum. T. lappaceum, T. diffusum, T. campestre, T. incarnatum and T. argutum showed only 44.8% similarity with other Trifolium species. Grouping together of accessions belonging to individual species indicated that incompatibility among species under study had restricted interspecific hybridization. Species belonging to subgenus Lotoidea clustered with species of subgenus Trifolium. Chonosemium species T. campestre formed one cluster with two Trifolium species T. hirtum and T. incarnatum. T. nigrescens was placed quite apart from the T. repens.
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Acknowledgments
Authors are thankful to the Director and Head of Crop Improvement Division, Indian Grassland and Fodder Research Institute, Jhansi, India, for encouragement and facilities. Thanks are also due to Indian Council of Agricultural Research, New Delhi, for providing financial support in form of AP Cess scheme. Authorities in National Bureau of Plant Genetic Resources, New Delhi, India; ILCA, Nairobi; IGPGR, Bulgaria; USDA, Georgia and IGER, UK are also thanked for providing seeds of different accessions.
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Malaviya, D.R., Roy, A.K., Kaushal, P. et al. Genetic similarity among Trifolium species based on isozyme banding pattern. Plant Syst Evol 276, 125–136 (2008). https://doi.org/10.1007/s00606-008-0070-7
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DOI: https://doi.org/10.1007/s00606-008-0070-7