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Evaluating the genetic relationship of Lilium species/cultivars based on target region amplification polymorphism (TRAP)

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Abstract

Most of the plant species from the genus Lilium L. have great ornamental and edible value. In this study, target region amplification polymorphism (TRAP) polymorphic marker technique was applied to assess the genetic relationships among 13 Lilium species/cultivars. The technique was also used to evaluate genetic diversity among two population of L. regale E. H. Wilson, cultivar of ‘Regale’ and ‘Regale Album’, and genetic diversity among two population of L. henryi Baker and cultivar of ‘Henryi’. In total, 221 fragments were amplified by 14 pairs of primers for 13 Lilium species/cultivars, of which 59 (26.70%) showed polymorphism. 1160 fragments were amplified for two population of L. regale, cultivar of ‘Regale’ and ‘Regale Album’, of which 943 (81.25%) were polymorphic. 798 fragments were amplified for two population of L. henryi and ‘Henryi’, of which 574 (71.93%) were polymorphic. A cluster analysis was performed based on UPGMA and PCoA by relying on the Nei’s genetic distance. Our results indicated that TRAP marker technique is informative and could be used to evaluate the genetic relationships among Lilium species/cultivars and genetic diversity among populations. The results will also provide more valuable information for Lilium resource protection and current plant breeding programs.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31601781) and Youth Research Fund of Beijing Academy of Agriculture and Forestry (QNJJ201910).

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Author notes

  1. Yue Hu and Jianwu Ren have contributed equally to this work.

Authors and Affiliations

  1. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China

    Yue Hu, Yixin Liu, Mingfang Zhang, The Su Moe, Mohammad Sayyar Khan, Yunpeng Du & Xiuhai Zhang

  2. Beijing Engineering Technology Research Center of Functional Floriculture, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing, 100097, China

    Yue Hu, Yixin Liu, Mingfang Zhang, The Su Moe, Mohammad Sayyar Khan, Yunpeng Du & Xiuhai Zhang

  3. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China

    Yue Hu & Jianwu Ren

  4. Pharmaceutical Research Laboratory, Biotechnology Research Department, Ministry of Education Mandalay Division, Kyaukse, 05151, Myanmar

    The Su Moe

  5. Genomics and Bioinformatics Laboratory, Institute of Biotechnology and Genetic Engineering (IBGE), Faculty of Crop Production Sciences, University of Agriculture, Peshawar, 25000, Pakistan

    Mohammad Sayyar Khan

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  1. Yue Hu
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Correspondence to Yunpeng Du or Xiuhai Zhang.

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Hu, Y., Ren, J., Liu, Y. et al. Evaluating the genetic relationship of Lilium species/cultivars based on target region amplification polymorphism (TRAP). Genet Resour Crop Evol 67, 503–513 (2020). https://doi.org/10.1007/s10722-019-00880-9

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  • DOI: https://doi.org/10.1007/s10722-019-00880-9

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