Abstract
Genetic changes in Siberian wild rye (Elymus sibiricus L.) germplasm conserved ex situ and with accelerated aging were studied using microsatellite markers. Five accessions, divided and deposited in the National Medium-term Genebank of Forage Germplasm of China (NMGFC) and the National Genebank of China (NGC) since 1998, were sampled. Four recently-regenerated accessions were selected to assess genetic changes under accelerated aging conditions using a thermostat-controlled cabinet at 45 °C for 7–13 days to obtain seeds with germination rates (Gr) of approximately 80%, 50%, and < 30%. Twenty-four simple sequence repeat (SSR) markers were used to screen 70 seeds of each sample and 137 SSR alleles in a naturally-aged germplasm set and 113 SSR alleles in an accelerated aging germplasm set were scored. Loss of alleles was observed with respect to the primer pair and sample in the NMGFC compared to that in the NGC, but no significant differences were found for each accession in seeds stored in NMGFC compared to that in seeds stored in NGC. Among-sample differences were the largest contributors to total SSR variation, at 70.6% and 76.2% for naturally-aged and accelerated-aging samples, respectively. Gr did not separate samples in either germplasm set based on genetic association analysis. Prominent changes in genetic diversity were found both in naturally-aged and accelerated-aging samples, with significant decreases in Shannon’s information index and Nei’s genetic diversity for cases with Gr ≤ 30% for both germplasm sets. These results should be valuable to improve the safe conservation of forage, especially for Elymus (family Gramineae).
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Acknowledgements
The authors would like to acknowledge Xinqiong Lu and Xia Xin from the Institute of Crop Science, Chinese Academy of Agricultural Sciences for kindly providing suggestions. We also thank the National Genebank of China and National Medium term Genebank of Forage of China for providing the seed samples. This work was supported by a grant from the Central Non-profit Research Institutes Fundamental Research Funds of China (1610332018020), Ministry of Agriculture Crop Germplasm Resources Protection and Utilization Project (2018NWB037), and the Natural Science Foundation of Inner Mongolia (2018BS03009).
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Zhiyong Li and Hongyan Li conceived and designed the experiment. Zhiyong Li supervised the entire study. Jun Li and Fan Huang performed the experiments. Fan Huang and Lei Liu analyzed the data. Wengui Shi and Hongyan Li prepared the materials. Fan Huang wrote the paper. All authors read and approved the final manuscript.
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Huang, F., Li, J., Li, H. et al. Assessment of genetic integrity of Siberian wild rye (Elymus sibiricus L.) germplasm conserved ex situ and under accelerated aging using microsatellite markers. Genet Resour Crop Evol 67, 367–379 (2020). https://doi.org/10.1007/s10722-019-00853-y
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DOI: https://doi.org/10.1007/s10722-019-00853-y