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Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)

  • Microsatellite Letters
  • Published:
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Abstract

Siberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai–Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.

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Acknowledgments

This work was supported by the Agricultural Science and Technology Achievements Transformation and Promotion Plan of Qinghai (2013-N-515) and the Joint Scholars project of The Dawn of West China Talent Training Program of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, 810008, China

    Yunting Lei, Yanyan Zhao, Feng Yu, Yuan Li & Quanwen Dou

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Yanyan Zhao, Feng Yu & Yuan Li

Authors
  1. Yunting Lei
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  2. Yanyan Zhao
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  3. Feng Yu
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  4. Yuan Li
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  5. Quanwen Dou
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Corresponding author

Correspondence to Quanwen Dou.

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Lei, Y., Zhao, Y., Yu, F. et al. Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.). Conservation Genet Resour 6, 861–864 (2014). https://doi.org/10.1007/s12686-014-0225-5

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  • DOI: https://doi.org/10.1007/s12686-014-0225-5

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