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Special HMW-GSs and their genes of Triticum turgidum subsp. dicoccoides accession D141 and the potential utilization in common wheat

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Abstract

High molecular weight glutenin subunits (HMW-GSs) are the critical components of wheat seed storage proteins. They play a crucial role in determining dough viscoelastic properties and end-use quality of wheat flour. To investigate the potential utilization of the special HMW-GSs from wild emmer wheat (2n = 4x = 28, AABB) accession D141 in common wheat (2n = 6x = 42, AABBDD), continued to our previous report of the 1Ay and its coding sequence, the other three HMW-GSs, 1Ax, 1Bx13.1 and 1By16.1, and their coding genes were isolated, characterized and transferred from D141. The 1Ax gene was silent because of containing a premature stop codon TGA at position 835–837. The ORFs of 1Bx13.1 and 1By16.1 were 2373 and 2157 bp in length, and encoded 789 and 717 amino acid residues, respectively. Compared with the other 20 Glu-1Bx alleles in GenBank, seven SNPs and one 18-bp deletion were detected in 1Bx13.1. Compared with the other 10 Glu-1By alleles in GenBank, nine SNPs were observed in 1By16.1. The mobility of 1Bx13.1 and 1By16.1 on the SDS-PAGE gel was most similar to that of 1Bx13 and 1By16 from common wheat (T. aestivum, 2n = 6x = 42, AABBDD) cv. ‘Jimai 20′, respectively. Protein secondary structure prediction showed that the combination of 1Bx13.1 + 1By16.1 possessed more α-helixes with more amino acid residues than that of 1Bx13 + 1By16. It suggested that 1Bx13.1 + 1By16.1 from wild emmer accession D141 might have more positive effects on bread-making quality than the superior subunit combination 1Bx13 + 1By16. The HMW-GS composition analysis of F1 and F2 between common wheat for female and wild emmer accession D141 for male, indicated that the special HMW-GSs from wild emmer accession D141 could be easily introduced into common wheat (2n = 42) by general inter-species hybridization.

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Abbreviations

aa:

Amino acid(s)

bp:

Base pair(s)

CTAB:

Cetyltrimethyl ammonium bromide

dNTP:

Deoxyribonucleoside triphosphate

HMW-GS:

High molecular weight glutenin subunit

InDel:

Insertion and deletion

IPTG:

Isopropyl β-Δ-thiogalactopyranoside

ORF:

Open reading frame

PCR:

Polymerase chain reaction

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SNP:

Single nucleotide polymorphism

References

  • Anderson OD, Greene FC (1989) The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid wheat. Theor Appl Genet 77:689–700

    Article  CAS  PubMed  Google Scholar 

  • Anjum FM, Khan MR, Din A, Saeed M, Pasha I, Arshad MU (2007) Wheat gluten: high molecular weight glutenin subunits—structure, genetics, and relation to dough elasticity. J Food Sci 72:R56–R63

  • Bi ZG, Wu BH, Hu XG, Guo XH, Liu DC, Zheng YL (2014) Identification of an active 1Ay gene from Triticum turgidum ssp. dicoccoides. Czech J Genet Plant Breed 50:208–215

    CAS  Google Scholar 

  • Ciaffi M, Lafiandra D, Porceddu E, Benedettellim S (1993) Storage protein variation in wild emmer wheat (Triticum turgidum ssp. dicoccoides) from Jordan and Turkey. I. Electrophoretic characterization of genotypes. Theor Appl Genet 86:474–480

    Article  CAS  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Forde J, Malpica JM, Halford NG, Shewry PR, Anderson OD, Greene FC, Miflin BJ (1985) The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Nucleic Acids Res 13:6817–6832

  • Gianibelli MC, Larroque OR, MacRichie F, Wrigley CW (2001) Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits. Cereal Chem 78:635–646

    Article  CAS  Google Scholar 

  • Guo XH, Bi ZG, Wu BH, Wang ZZ, Hu JL, Zheng YL, Liu DC (2013a) ChAy/Bx, a novel chimeric high-molecular-weight glutenin subunit gene apparently created by homoeologous recombination in Triticum turgidum ssp. dicoccoides. Gene 531:318–325

    Article  CAS  PubMed  Google Scholar 

  • Guo XH, Wu BH, Hu XG, Bi ZG, Wang ZZ, Liu DC, Zheng YL (2013b) Molecular characterization of two y-type high molecular weight glutenin subunit alleles 1Ay12 * and 1Ay8 * from cultivated einkorn wheat (Triticum monococcum ssp. monococcum). Gene 516:1–7

    Article  CAS  PubMed  Google Scholar 

  • Halford NG, Field JM, Blair H, Urwin P, Moore K, Robert L, Thompson R, Flavell RB, Tatham AS, Shewry PR (1992) Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83:373–378

    Article  CAS  PubMed  Google Scholar 

  • Hu XG, Wu BH, Yan ZH, Liu DC, Wei YM, Zheng YL (2010) Characterization of a Novel 1Ay Gene and Its Expression Protein in Triticum urartu. Agr Sci China 9:1543–1552

    Article  CAS  Google Scholar 

  • Hu XG, Wu BH, Bi ZG, Liu DC, Zhang LQ, Yan ZH, Wei YM, Zheng YL (2012) Allelic variation and distribution of HMW glutenin subunit 1Ay in Triticum species. Genet Resour Crop Ev 59:491–497

    Article  CAS  Google Scholar 

  • Jiang QT, Wei YM, Wang F, Wang JR, Yan ZH, Zheng YL (2009) Characterization and comparative analysis of HMW glutenin 1Ay alleles with differential expressions. BMC Plant Biol 9:16

    Article  PubMed  PubMed Central  Google Scholar 

  • Jin M, Xie ZZ, Ge P, Li J, Jiang SS, Subburaj S, Li XH, Zeller FJ, Hsam SL, Yan YM (2012) Identification and molecular characterisation of HMW glutenin subunit 1By16 * in wild emmer. J Appl Genet 53:249–258

    Article  CAS  PubMed  Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  • Levy AA, Galili G, Feldman M (1988) Polymorphism and genetic control of high molecular weight glutenin subunits in wild tetraploid wheat Triticum turgidum var. dicoccoides. Heredity 61:63–72

    Article  CAS  Google Scholar 

  • Margiotta B, Urbano M, Colaprico G, Turchetta T, Lafiandra D (1998) Variation of high molecular weight glutenin subunits in tetraploid wheats of genomic formula AAGG. In: Slinkard AE (ed) Proceedings of the 9th international wheat genetics symposium. University Extension Press, Saskatoon, pp 195–197

    Google Scholar 

  • McGuffin LJ, Bryson K, Jones DT (2000) The PSIPRED protein structure prediction server. Bioinformatics 16:404–405

    Article  CAS  PubMed  Google Scholar 

  • Nevo E, Payne PI (1987) Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel. 1. Geographical patterns and ecological predictability. Theor Appl Genet 74:827–836

    Article  CAS  PubMed  Google Scholar 

  • Nevo E, Korol AB, Beiles A, Fahima T (2002) Evolution of wild emmer and wheat improvement: population genetics, genetic resources, and genome organization of wheat’s progenitor. Springer, Triticum dicoccoides

  • Pang BS, Zhang XY (2008) Isolation and molecular characterization of high molecular weight glutenin subunit genes 1Bx13 and 1By16 from hexaploid wheat. J Integr Plant Biol 50:329–337

    Article  CAS  PubMed  Google Scholar 

  • Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38:141–153

    Article  CAS  Google Scholar 

  • Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the highmolecular-weight subunits of wheat glutenin. Part 1: allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–236

  • Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci. Glu-A1, Glu-B1 and Glu-D1 which code for high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35

    Google Scholar 

  • Ribeiro M, Bancel E, Faye A, Dardevet M, Ravel C, Branlard G, Igrejas G (2013) Proteogenomic characterization of novel x-type high molecular weight glutenin subunit 1Ax1.1. Int J Mol Sci 14:5650–5667

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shewry PR, Tatham AS (1997) Disulphide bonds in wheat gluten proteins. J Cereal Sci 25:207–227

    Article  CAS  Google Scholar 

  • Shewry PR, Halford NG, Tatham AS (1992) High molecular weight subunits of wheat glutenin. J Cereal Sci 15:105–120

    Article  CAS  Google Scholar 

  • Shewry PR, Napier JA, Tatham AS (1995) Seed storage proteins: structures and biosynthesis. Plant Cell 7:945–956

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shewry PR, Halford NG, Belton PS, Tatham AS (2002) The structure and properties of gluten: an elastic protein from wheat grain. Philos Trans R Soc Lond B Biol Sci 357:133–142

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shewry PR, Gilbert SM, Savage AWJ, Tatham AS, Wan YF, Belton PS, Wellner N, D’Ovidio R, Békés F, Halford NG (2003) Sequence and properties of HMW subunit 1Bx20 from pasta wheat (Triticum durum) which is associated with poor end use properties. Theor Appl Genet 106:744–750

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson RD, Bartels D, Harberd NP (1985) Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res 13:6833–6846

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wan Y, Wang D, Shewry PR, Halford NG (2002) Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet 104:828–839

    Article  CAS  PubMed  Google Scholar 

  • Xie W, Nevo E (2008) Wild emmer: genetic resources, gene mapping and potential for wheat improvement. Euphytica 164:603–614

    Article  Google Scholar 

  • Xie RL, Wan YF, Zhang Y, Wang DW (2001) HMW glutenin subunits in multiploid Aegilops species: composition analysis and molecular cloning of coding sequences. Chin Sci Bull 46:309–313

    Article  CAS  Google Scholar 

  • Xu LL, Li W, Wei YM, Zheng YL (2009) Genetic diversity of HMW glutenin subunits in diploid, tetraploid and hexaploid Triticum species. Genet Resour Crop Evol 56:377–391

  • Xu SS, Khan K, Klindworth DL, Faris JD, Nygard G (2004) Chromosomal location of genes for novel glutenin subunits and gliadins in wild emmer wheat (Triticum turgidum L. var. dicoccoides). Theor Appl Genet 108:1221–1228

  • Yu WL, Li GJ, Wu BH, Zheng YL, Liu DC, Yan ZH (2005) Variation of high-molecular-weight glutenin subunits in Triticum dicoccoides and its potential utilization in common wheat. J Sichuan Agr Univ 23: 252-257 (in Chinese, with English abstract)

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    Authors and Affiliations

    1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

      Xiao-Hui Guo, Ji-Liang Hu, Bi-Hua Wu, Zhen-Zhen Wang, Dong Wang, Deng-Cai Liu & You-Liang Zheng

    2. College of Food and Biological Engineering, Xuchang University, Xuchang, 461000, China

      Xiao-Hui Guo

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    1. Xiao-Hui Guo
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    Correspondence to Bi-Hua Wu.

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    Xiao-Hui Guo and Ji-Liang Hu have contributed equally to this work.

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    Guo, XH., Hu, JL., Wu, BH. et al. Special HMW-GSs and their genes of Triticum turgidum subsp. dicoccoides accession D141 and the potential utilization in common wheat. Genet Resour Crop Evol 63, 833–844 (2016). https://doi.org/10.1007/s10722-015-0287-6

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