Abstract
We previously found a specific DNA fragment, designated Band 1, in the cytoplasm of a Mexican hexaploid wild potato species, Solanum demissum, and varieties with the S. demissum cytoplasm. In this study, we show that Band 1 also occurs substoichiometrically in various species and varieties. The S. demissum DNA sequence harboring Band 1 was extended by genome walking to 7,040 bp, and whole-genome sequencing of the S. verrucosum genome generated a 10,794-bp contig with the corresponding sequence. Their 3′-terminal end sequences had 100 % homology with segment 2 of the S. tuberosum mitochondrial genome, proving that Band 1 originated from a recombination-derived mitochondrial genome. Both normal and recombination-derived mitochondrial genomes co-existed in Band 1 carriers. The presence or absence of Band 1 was surveyed for four accessions of tomato and wild relatives and 172 accessions of 38 Solanum species or potato and tuber-bearing wild relatives, mostly from Mexican species. Together, with the results of our previous survey (mostly for South American species), we conclude that Band 1 is retained stoichiometrically only among S. verrucosum and Mexican polyploid species, supporting S. verrucosum as a maternal ancestor for all Mexican polyploid species. The presence or absence of Band 1 was not uniform within these species and was not associated with ploidy, geographical distribution, or latitude. Several evolutionary hypotheses to explain intraspecific variation were discussed.
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Acknowledgments
We thank the US Potato Genebank (NRSP-6), Sturgeon Bay, Wisconsin, for providing the Solanum seeds used in this study, and Dr. David M. Spooner for kindly checking taxonomic identities with his updated taxonomy and reviewing the manuscript. We also thank Dr. Itsuro Sugimura and Naoyuki Sugimoto, Hokkaido System Science Co. Ltd., for initial handling of Illumina data, and Shizuka Souma for technical assistance. This study was supported by Calbee, Inc. and Calbee Potato, Inc.
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Sanetomo, R., Hosaka, K. A recombination-derived mitochondrial genome retained stoichiometrically only among Solanum verrucosum Schltdl. and Mexican polyploid wild potato species. Genet Resour Crop Evol 60, 2391–2404 (2013). https://doi.org/10.1007/s10722-013-0007-z
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DOI: https://doi.org/10.1007/s10722-013-0007-z