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Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits

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Abstract

The high molecular weight glutenin subunits (HMW-GS) can be used for wheat quality improvement. Two novel alleles (designated 1Dx1.5* and 1Dy12.2*, respectively) at the Glu-D1 locus were identified in the Chinese wheat landrace variety ‘Jiuquanjinbaoyin’ by comparison of subunit mobility with that previously identified in several standard hexaploid wheats. The 1Dx1.5* and 1Dy12.2* genes were isolated using the allele-specific PCR primers and the complete open reading frames (ORFs) were obtained. Allele 1Dx1.5* consists of 2487 bp encoding a mature protein of 827 amino acid residues, whereas 1Dy12.2* consists 1980 bp encoding 658 residues. Comparisons of amino acid sequences analysis showed that 1Dx1.5* had higher similarity with the HMW-GS isolated from the wheat related species (Aegilops tauchi Coss.) than from the bread wheat varieties (Triticum aestivum L.). The 1Dy12.2* amino acid sequence showed a generally similar to the 1Dy12* isolated from Chinese endemic wheats. Meanwhile, the dough properties of the lines expressing (null, 7+8, 1.5*+10), (null, 7+8, 2+12.2*) and (null, 7+8, 2+12), respectively, were measured by a Mixograph, which demonstrates that the alleles 1.5*+10 can be considered as having positive impact on dough strength when compared with the alleles 2+12. In addition, the subunits 2+12.2* also showed a greater impact on dough strength than 2+12.

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Acknowledgments

This research is supported by grants from the National Basic Research Program of China (2006CB100201).

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Authors and Affiliations

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Xiaomin Guo, Junxian Guo, Xiuquan Li, Xinming Yang & Lihui Li

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  1. Xiaomin Guo
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  2. Junxian Guo
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  3. Xiuquan Li
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  4. Xinming Yang
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  5. Lihui Li
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Correspondence to Lihui Li.

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Guo, X., Guo, J., Li, X. et al. Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits. Genet Resour Crop Evol 57, 1217–1225 (2010). https://doi.org/10.1007/s10722-010-9561-9

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