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The interaction of folate-modified Bletilla striata polysaccharide-based micelle with bovine serum albumin

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Abstract

We fabricated an amphiphilic folate-modified Bletilla striata polysaccharide (FA-BSP-SA) copolymer that exhibited good biocompatibility and superior antitumor effects. This study investigated the affinity between FA-BSP-SA and bovine serum albumin (BSA) via multispetroscopic approaches. Changes in the morphology and particle size showed that FA-BSP-SA formed a blurry “protein corona”. Stern–Volmer equation demonstrated that FA-BSP-SA micelles decreased the fluorescence of BSA via static quenching. The measurement results of thermodynamic parameters (entropy change, enthalpy change, and Gibbs free energy) suggested that the binding between FA-BSP-SA and BSA was spontaneous in which Van der Waals forces and hydrogen bonding played major roles. The results from synchronous fluorescence, circular dichroism, and UV spectra also revealed that BSA conformation was slightly altered by decreasing α-helical contents. In addition, the antitumor effects in vitro of Dox@FA-BSP-SA micelles and the cellular uptake behavior of micelles in 4T1 cells were decreased after incubating with BSA.

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Acknowledgements

The research was supported by Graduate Innovation Fund of Jilin University (101832018C084).

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  1. Xinying Wang and Guangyuan Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmaceutics, School of Pharmacy, Jilin University, No. 1266, Fujin Road, Changchun, 130021, China

    Xinying Wang, Guangyuan Zhang, Di Yu, Ning Wang & Qingxiang Guan

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  1. Xinying Wang
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  2. Guangyuan Zhang
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Contributions

Xinying Wang: Conceptualization, Methodology, Formal analysis, Writing—Original Draft, Writing—Review & Editing. Guangyuan Zhang: Conceptualization, Software, Data Curation, Writing—Review & Editing, Visualization, Di Yu: Validation, Investigation, Resources. Ning Wang: Validation, Data Curation, Writing—Review & Editing. Qingxiang Guan: Conceptualization, Writing—Review & Editing, Supervision, Funding acquisition.

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Correspondence to Qingxiang Guan.

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Wang, X., Zhang, G., Yu, D. et al. The interaction of folate-modified Bletilla striata polysaccharide-based micelle with bovine serum albumin. Glycoconj J 38, 585–597 (2021). https://doi.org/10.1007/s10719-021-10022-y

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