Abstract
The interaction between silver nanoparticles (SNPs) and Bovine Serum Albumin (BSA) was investigated at physiological pH in an aqueous solution using fluorescence spectroscopy. The analysis of fluorescence spectrum and fluorescence intensity indicates that SNPs have a strong ability to quench the intrinsic fluorescence of BSA by both static and dynamic quenching mechanisms. Resonance light scattering (RLS) spectra indicated the formation of a complex between BSA and SNP. The number of binding sites ‘n’ and binding constants ‘K’ were determined at different temperatures based on fluorescence quenching. The thermodynamic parameters namely ∆H°, ∆G°, ∆S° were calculated at different temperatures and the results indicate that hydrophobic forces are predominant in the SNP-BSA complex. Negative ∆G° values imply that the binding process is spontaneous. Synchronous fluorescence spectra showed a blue shift which is indicative of increasing hydrophobicity.
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Acknowledgements
We gratefully acknowledge the Department of Science and Technology (DST), Government of India, for their financial support through the DST INSPIRE - AORC scheme. We would also like to thank Mr. Narayan Karmakar, Project fellow, National Centre for Nanomaterials & Nanotechnology, University of Mumbai for the DLS spectrum.
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Mariam, J., Dongre, P.M. & Kothari, D.C. Study of Interaction of Silver Nanoparticles with Bovine Serum Albumin Using Fluorescence Spectroscopy. J Fluoresc 21, 2193–2199 (2011). https://doi.org/10.1007/s10895-011-0922-3
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DOI: https://doi.org/10.1007/s10895-011-0922-3