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Nucleotide diversity of a ND5 fragment confirms that population expansion is the most suitable explanation for the mtDNA haplotype polymorphism of Drosophila subobscura

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Abstract

Results from mitochondria (mt) DNA restriction site analyses (RSAs) have revealed that wild populations of Drosophila subobscura are formed by two common (I and II) and some rare, often endemic, low-frequency haplotypes. In the study reported here, we analysed nucleotide diversity in a 942-bp fragment of the mtDNA ND5 gene in 48 D. subobscura individuals captured from three populations that showed haplotypes I, II or the less common ones, as well as in one additional individual belonging to D. guanche that was taken as an outgroup. RSAs and sequencing results were compared. The two approaches yielded similar nucleotide variability parameters, suggesting a consistency in the results obtained from mtDNA dynamics in natural populations of D. subobscura. Patterns of polymorphism at ND5 are most consistent with the hypothesis of population expansion after a bottleneck that may have occurred since the last glaciation or which may occur seasonally after the summer and winter. However, we cannot rule out that selection has a role in maintaining the two major haplotypes at intermediate frequencies in worldwide populations of D. subobscura.

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Acknowledgments

This work was supported by grants PB96-0793 and BOS2000-1000 from the Dirección General de Enseñanza Superior (Ministerio de Educación y Cultura, Spain).

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Authors and Affiliations

  1. Laboratori de Genètica, Departament de Biologia, Edifici Guillem Colom, Facultat de Ciències, Universitat de les Illes Balears, Campus de la UIB, Palma de Mallorca, 07122, Balearic Islands, Spain

    José A. Castro, Antònia Picornell & Maria Misericòrdia Ramon

  2. Institut “Cavanilles” de Biodiversitat i Biologia Evolutiva, Universitat de València, Apartado de Correos 22085, 46071, Valencia, Spain

    Eladio Barrio, Ana González & Andrés Moya

  3. Centre Superior d’ Investigació en Salut Pública (CSISP), Av. de Catalunya 21, 46020, Valencia, Spain

    Andrés Moya

  4. CIBER en Epidemiología y Salud Pública (CIBEResp), Valencia, Spain

    Andrés Moya

Authors
  1. José A. Castro
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  2. Eladio Barrio
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  3. Ana González
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  4. Antònia Picornell
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  5. Maria Misericòrdia Ramon
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  6. Andrés Moya
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Corresponding author

Correspondence to José A. Castro.

Appendix I

Appendix I

See Table 8.

Table 8 Polymorphic nucleotide sites in Drosophila subobscura and D. guanche in the analysed region of the ND5 gene
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Castro, J.A., Barrio, E., González, A. et al. Nucleotide diversity of a ND5 fragment confirms that population expansion is the most suitable explanation for the mtDNA haplotype polymorphism of Drosophila subobscura . Genetica 138, 819–829 (2010). https://doi.org/10.1007/s10709-010-9464-x

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  • DOI: https://doi.org/10.1007/s10709-010-9464-x

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