Abstract
We studied the effects of Yarrowia lipolytica biomass on digestive enzymes, blood biochemical profile, energy metabolism enzymes, and proximate meat composition of Nile tilapias. The experiment was entirely randomized with four replications. The animals (n = 20 per repetition) were fed with 0%, 3%, 5%, and 7% of biomass for 40 days and then blood and liver were analyzed. There was an increase in the activities of chymotrypsin (5, 7% groups), trypsin (3, 5% groups), and sucrase (7% group) compared to the respective control groups. On the other hand, maltase activity was significantly reduced for all yeast biomass treatments, while the supplementation did not influence lipase and amylase activities. Moreover, the blood triacylglycerol concentrations were increased in the 7% group, while any treatment modified blood total cholesterol, glycemia, and hepatic glycogen content. Y. lipolytica biomass promoted significant increases in meat protein and lipid contents without changes in moisture and ash parameters. Furthermore, Y. lipolytica biomass promoted increases in hexokinase (3% group), phosphofructokinase (5, 7% groups), glucose-6-phosphate dehydrogenase (5% group), citrate synthase (3% group), aspartate aminotransferase and alanine aminotransferase (3% group), and glutamate dehydrogenase (3, 5% groups) compared to the respective control groups. At the same time, no changes were observed in the activity of glucose-6-phosphatase. Y. lipolytica biomass supplementation in tilapias’ diet can modulate the digestive system and improve nutrient disponibility to the cells. Moreover, the changes in the metabolic profile and in energy metabolism can be correlated with the improvement of meat composition. Therefore, the Y. lipolytica biomass has a great potential to be used as a feed ingredient for Nile tilapias.
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Acknowledgements
The authors are grateful to Professor Patricia Valente from the Federal University of Rio Grande do Sul (UFRGS, Brazil) for donating the Yarrowia lipolytica QU69 strain and Professor Thiago Bergler Bitencourt from the Federal University of Fronteira Sul by providing the Yarrowia lipolytica–fermented biomass.
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This work was supported by Federal University of Fronteira Sul and Fundação Araucária [grant no. 739/GR/UFFS/2019 - Concession of social inclusion scholarships (PIBIS) - Fundação Araucária].
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All authors contributed to the study conception and design. Material preparation, conceptualization, data collection, and analysis were performed by Milena Cia Retcheski, Luiz Vitor Maximowski, Keveen Jhonathan Soares Escorsin, Jéssica Kimie de Almeida Rosa Kurosaki, Silvia Romão, Thiago Bergler Bitencourt, Jorge Erick Garcia Parra, Luisa and Helena Cazarolli. The first draft of the manuscript was written by Luisa Helena Cazarolli and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Retcheski, M.C., Maximowski, L.V., Escorsin, K.J.S. et al. Yarrowia lipolytica biomass—a potential additive to boost metabolic and physiological responses of Nile tilapia. Fish Physiol Biochem 49, 655–670 (2023). https://doi.org/10.1007/s10695-023-01219-z
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DOI: https://doi.org/10.1007/s10695-023-01219-z