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Dietary supplementation of multi-strain probiotic in male rainbow trout (Oncorhynchus mykiss) broodstock: Effects on feed efficiency, hemato-biochemical parameters, immune response, and semen quality

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Abstract

The present study aimed to determine the effects of dietary probiotic supplementation on feed efficiency, physiological parameters, and semen quality of male rainbow trout (Oncorhynchus mykiss) broodstock. For this purpose, a total of 48 breeders with an average initial weight of 1366.1 ± 33.8 g were divided into 4 groups and 3 replicates. Fish were fed with diets containing 0 (control), 1 × 109 (P1), 2 × 109 (P2), and 4 × 109 (P3) CFU multi-strain probiotic kg−1 diet for 8 weeks. According to the results, P2 treatment significantly enhanced body weight increase, specific growth rate, and protein efficiency ratio and decreased feed conversion ratio. Moreover, the highest values of red blood cells count, hemoglobin, and hematocrit values were observed in P2 treatment (P < 0.05). The lowest levels of glucose, cholesterol, and triglyceride were found in P1, P2, and P3 treatments, respectively. Also, the highest levels of total protein and albumin were obtained in P2 and P1 treatments (P < 0.05). Based on the results, plasma enzymes contents were significantly decreased in P2 and P3 treatments. In terms of immune parameters, the complement component 3, complement component 4, and immunoglobulin M levels were increased in all probiotic-fed treatments (P < 0.05). For spermatological features, the highest spermatocrit value, sperm concentration, and motility time were observed in the P2 treatment (P < 0.05). Consequently, we conclude that multi-strain probiotics can be used as functional feed additives in male rainbow trout broodstock to enhance semen quality, improve physiological responses, and better feed efficiency.

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The data and materials that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The researchers would like to thank T. Shamsipour for providing broodstock and ponds for this project. We would also like to express our thanks to M. Mohammadi and other friends who contributed to advance this experiment.

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Authors and Affiliations

  1. Fisheries Department, Faculty of Natural Resources, University of Guilan, P. O. Box 1144, Sowmeh Sara, Guilan, Iran

    Erfan Akbari Nargesi & Bahram Falahatkar

  2. Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Rasht, Guilan, Iran

    Bahram Falahatkar

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  1. Erfan Akbari Nargesi
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Contributions

Erfan Akbari Nargesi: conceptualization, methodology, acquisition of data, software analysis, interpretation of data, writing-original draft preparation, reviewing, and editing; Bahram Falahatkar: conceptualization, supervision, methodology, reviewing, and editing.

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Correspondence to Bahram Falahatkar.

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All experiments were conducted in accordance with the National Institutes of Health guide for the care and use of laboratory animals (National Research Council 2011). The study was approved by the Research Ethics Committee of the University of Guilan.

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Akbari Nargesi, E., Falahatkar, B. Dietary supplementation of multi-strain probiotic in male rainbow trout (Oncorhynchus mykiss) broodstock: Effects on feed efficiency, hemato-biochemical parameters, immune response, and semen quality. Fish Physiol Biochem 49, 371–384 (2023). https://doi.org/10.1007/s10695-023-01181-w

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