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QTL mapping and candidate gene analysis of microspore embryogenesis in Capsicum

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Abstract

Microspore embryogenesis is widely used in plant breeding to obtain homozygous lines. It is an important way of obtaining haploids and it is valuable for QTL (Quantitative Trailt Locus) mapping and genetic research. We analyzed the microspore embryogenesis ability of 142 recombinant inbred lines (RIL) and mapped QTLs for microspore embryogenesis based on a genetic map constructed using specific-locus amplified fragment sequencing SLAF-seq technology. Results revealed that microspore embryogenesis ability in pepper is a quantitative character. Seven QTLs were detected on linkage groups LG2, LG6, LG8, LG11 and LG12. The peak logarithm of odds (LOD) score of the seven QTLs ranged from 3.51 to 6.66, and the range of phenotypic variation explained by a single QTL ranged from 4.76 to 18.30%. We identified a major QTL, Me2.2, located in the region of 116.5–118.5 cM on LG2; the corresponding physical segment was 166,621,667–167,038,758 bp, and 24 candidate genes were predicted in this region. Functional annotation indicated that these genes are closely related to the initiation of cell division, symmetrical or asymmetric cell division, and other life processes. The study laid a good foundation for further research on the mechanism of microspore embryogenesis.

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Data availability

All data generated or analysed during this study are included in this published article and its supplementary information files.

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Not applicable.

Abbreviations

QTL:

Quantitative trait locus

RIL:

Recombinant inbred lines

SLAF-seq:

Specific-locus amplified fragment sequencing

LOD:

Logarithm of odds

GO:

Gene ontology

References

  • Burnett L, Yarrow S, Huang B (1992) Embryogenesis and plant regeneration from isolated microscope culture of Brassica napus L.ssp.oleifera. Plant Cell Rep 11(8):215–218

    CAS  Google Scholar 

  • Chaim AB, Paran I, Grube RC, Jahn M, Wijk R, Peleman J (2001) QTL mapping of fruit-related traits in pepper (Capsicum annuum). Theor Appl Genet 102(6–7):1016–1028

    Article  CAS  Google Scholar 

  • Cogan N, Harvey E, Robinson H, Lynn J, Pink D, Newbury HJ, Puddephat I (2001) The effect of anther culture and plant genetic background on Agrobacterium rhizogenes-mediated transformation of commercial cultivars and derived doubled-haploid Brassica oleracea. Plant Cell Rep 20(8):755–762

    Article  CAS  Google Scholar 

  • Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8(4):377–424

    Article  CAS  Google Scholar 

  • Dwivedi SL, Britt AB, Tripathi L, Sharma S, Upadhyaya HD, Ortiz R (2015) Haploids: constraints and opportunities in plant breeding. Biotechnol Adv 33(6):812–829

    Article  Google Scholar 

  • Forster BP, Heberle BE, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12(8):368–375

    Article  CAS  Google Scholar 

  • Gilles LM, Khaled A, Laffaire JB, Chaignon S, Gendrot G, Laplaige J, Beydon G, Bayle V, Barret P, Comadran J, Martinant JP, Rogowsky MP, Widiez T (2017) Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize. EMBO J 36:707–717

    Article  CAS  Google Scholar 

  • Hosp J, Maraschin SF, Touraev A, Boutilier K (2007) Functional genomics of microspore embryogenesis. Euphytica 158(3):275–285. https://doi.org/10.1007/s10681-006-9238-9

    Article  Google Scholar 

  • Hur YS, Um JH, Kim S, Park HJ, Lim JS, Kim WY, Jun SE, Yoom EK, Lim J, Ohme TM, Kim D, Park J, Kim GT, Chon CI (2015) Arabidopsis thaliana homeobox 12 (ATHB12), a homeodomain-leucine zipper protein, regulates leaf growth by promoting cell expansion and endoreduplication. New Phytol 205(1):316–328

    Article  CAS  Google Scholar 

  • Kelliher T, Starr D, Richbourg L, Chintamanani S, Delzer B, Nuccio M, Green J, Chen ZY, McCuiston J, Wang WL, Liebler T, Bullock P, Martin B (2017) MATRILINEAL, a sperm-specific phospholipase, triggers maize haploid induction. Nature 542:105–109

  • Kim M, Jang IC, Kim JA, Oark AJ, Yoon MC, Lee YW (2008) Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant Cell Rep 27(3):425–434

    Article  CAS  Google Scholar 

  • Kim S, Park M, Yeom SI, Kim YM, Lee JM, Lee HA, Seo EY, Choi JY, Cheong KC, Kim KT, Jung KY, Lee GW, Oh SK, Bae CY, Kim SB, Lee HY, Kim SY, Kim MS, Kang BC, Jo YD, Yang HB, Jeong HJ, Kang WH, Kwon JK, Shin C, Lim JY, Park JH, Huh JH, Kim JS, Kim BD, Cohen O, Paran I, Suh MC, Lee SB, Kim YK, Shin Y (2014) Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species. Nat Genet 46(3):270–278

    Article  CAS  Google Scholar 

  • Kumar A, Kanwal P, Gupta AK, Singh BR, Gaur VS (2014) A full-length Dof1 transcription factor of finger millet and its response to a circadian cycle. Plant Mol Biol Report 32(2):419–427

    Article  CAS  Google Scholar 

  • Lantos C, Juhász AG, Somogyi G, Ötvös K, Vági P, Mihály R, Kristóf Z, Somogyi N, Pauk J (2009) Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat. Plant Cell Tissue Organ C 97(3):285–293

    Article  Google Scholar 

  • Lei M, Li HH, Zhang LY, Wang JK (2015) QTL IciMapping: integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J 3(3):269–283

    Article  Google Scholar 

  • Li HC, Devaux P (2003) High frequency regeneration of barley doubled haploid plants from isolated microspore culture. Plant Sci 164:379–386

    Article  CAS  Google Scholar 

  • Li CL, Tong XR, Zhu ZQ, Gu SR, Jiang ZR, Zhang SG (2008) Androgenesis and embryogenesis in isolated microspore culture of Capsicum annuum L. Acta Hortic Sin 35(11):1613–1620

    Google Scholar 

  • Liu WG, Zheng MY, Polle AE, Konzak FC (2002) Highly efficient doubled-haploid production in wheat (Triticum aestivum L.) via induced microspore embryogenesis. Crop Sci 42:686–692

    Google Scholar 

  • Liu CX, Li X, Meng DX, Chen C, Dong X, Xu XW, Chen BJ, Li W, Li L, Tian XL, Zhao HM, Song WB, Luo HS, Zhang QH, Lai JS, Jin WW, Yan JB, Chen SJ (2017) A 4-bp insertion at ZmPLA1 encoding a putative phospholipase a generates haploid induction in maize. Mol Plant 10:520–522

    Article  CAS  Google Scholar 

  • Maraschin DFS, Caspers M, Potokina E, Graner A, Spaink PH, Wang M (2006) cDNA array analysis of stress-induced gene expression in barley androgenesis. Physiol Plant 127(4):535–550

    Article  CAS  Google Scholar 

  • Murovec J, Bohanec B (2012). Haploids and doubled haploids in plant breeding. (eds) Plant breeding, 1: 88–102. ISBN 978–956–307–932–5

  • Ouyang J.W., Jia S., Zhang C., Chen X., Feng G., (1989). A new synthetic medium (W14 medium) wheat anther culture. Annual report for 1988 institute of genetics,academica sinica,Beijing, 1989: 102.

  • Ravi M, Chan SW (2010) Haploid plants produced by centromere-mediated genome elimination. Nature 464:615–618

    Article  CAS  Google Scholar 

  • Sánchez-Díaz RA, Castillo AM, Vallés MP (2013) Microspore embryogenesis in wheat: new marker genes for early, middle and late stages of embryo development. Plant Reprod 26(3):287–296

    Article  Google Scholar 

  • Seguisimarro JM (2015) Doubled haploidy in model and recalcitrant species. Front Plant Sci 6:1175

    Google Scholar 

  • Seifert F, Bössow S, Kumlehn J, Gnad H, Scholten S (2016) Analysis of wheat microspore embryogenesis induction by transcriptome and small RNA sequencing using the highly responsive cultivar “Svilena.” BMC Plant Biol 16(1):1–16. https://doi.org/10.1186/s12870-016-0782-8

    Article  CAS  Google Scholar 

  • Sondergaard TE, Schulz A, Palmgren MG (2004) Energization of transport processes in plants. Roles of the plasma membrane H+-ATPase1. Plant Physiol 136(1):2475–2482

    Article  CAS  Google Scholar 

  • Swanson EB (1990) Microspore culture in Brassica. Plant Cell Tissue C 6:159–169

    Article  CAS  Google Scholar 

  • Tsukazaki H, Kuginuki Y, Aida R, Suzuki T (2002) Agrobacterium-mediated transformation of a doubled haploid line of cabbage. Plant Cell Rep 21(3):257–262

    Article  CAS  Google Scholar 

  • Vrinten PL, Nakamura T, Kasha KJ (1999) Characterization of cDNAs expressed in the early stages of microspore embryogenesis in barley (Hordeum vulgare L.) L. Plant Mol Biol 41(4):455–463

    Article  CAS  Google Scholar 

  • Xiang L, Meng D, Chen SJ, Luo HS, Zhang QH, Jin WW, Yan JB (2017) Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction. Nat Commun 8(1):368–375

    Google Scholar 

  • Zeng AS, Yan JY, Song LX, Gao B, Zhang YX, Li JQ, Liu HH, Hou XL, Li Y (2015) Induction and development of microspore-derived embryos in broccoli×white-headed cabbage hybrids microspore culture. Euphytica 203(2):261–272

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by General Program of National Natural Science Foundation of China (32072566), the Program of Beijing Municipal Science and Technology Committee (Z191100004019010-4), Innovation Ability Construction Project of Beijing Academy of Agriculture and Forest Sciences (KJCX20200113). Funding bodies did not take part in study design and collection, analysis, interpretation of data, and writing the manuscript.

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Authors and Affiliations

  1. Vegetable Research Center of Beijing Academy of Agricultural and Forestry Sciences, Zhanghua Road 50, Beijing, 100081, China

    Jingyi Bai, Bin Chen, Meixia Qi, Heshan Du, Sansheng Geng & Xiaofen Zhang

  2. College of Horticulture, China Agricultural University, Yuanmingyuan West Road 2, Beijing, 100083, China

    Meixia Qi & Qian Wang

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  1. Jingyi Bai
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  2. Bin Chen
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  3. Meixia Qi
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  4. Heshan Du
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  5. Qian Wang
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  6. Sansheng Geng
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  7. Xiaofen Zhang
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Contributions

XFZ and SSG contributed to the experimental design. QMX performed experiments, analyzed results. MHS, TTD, GYW and JYB participated in manuscript writing. MHS, GYW and YXW participated in experimental design and statistical analysis. All authors read and approved the final manuscript.

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Correspondence to Sansheng Geng or Xiaofen Zhang.

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Bai, J., Chen, B., Qi, M. et al. QTL mapping and candidate gene analysis of microspore embryogenesis in Capsicum. Euphytica 219, 3 (2023). https://doi.org/10.1007/s10681-022-03100-1

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