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GG13: a candidate gene related to seed development and viability from apomictic Paspalum notatum

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Abstract

One way to transfer apomixis (asexual reproduction through seeds) to major crops could be through genetic transformation, but for this a set of transgenes that triggers each of the involved processes should be generated. Although most angiosperms require an endospermic balance number (EBN) for normal endosperm development, tetraploid Paspalum notatum is EBN- insensitive. Here, a candidate gene (GG13) of Paspalum notatum associated with endosperm development in insensitive EBN crosses was analyzed by gene silencing in Arabidopsis thaliana. When comparing the silenced and control conditions, silenced condition evidenced less relative expression in RT-qPCR experiments, more abortions (6 vs. 1), fewer seeds (16 vs. 34), a more elongated shape (shape index 1.83 vs. 1.57), and lower germination (4 vs. 36 %) in phenotypic analyses. The above results were consistent with bioinformatically predicted functions for GG13 gene. Therefore, the importance of the GG13 gene in endosperm development in EBN-insensitive seeds was demonstrated, offering the possibility of incorporating apomixis into major crops.

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Acknowledgements

We thank Ph.D. John W. Miles for his valuable advice and discussions. This work was financed by the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) grant PIP-11220090100613 and P-UE: 22920160100043CO (IICAR), the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) grant PICT-2012-1321, the Secretaría de Ciencia y Técnica, Universidad Nacional de Rosario (UNR) grants AGR233 y AGR317. CA Acuña and SA Felitti are research career members of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). CL Quarin is a professor in the Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste. The authors declare that they have no conflict of interest.

Funding

This work was financed by the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) grant PIP-11,220,090,100,613 and P-UE: 22920160100043CO (IICAR), the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) grant PICT-2012-1321, the Secretaría de Ciencia y Técnica, Universidad Nacional de Rosario (UNR) grants AGR233 y AGR317.

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Authors and Affiliations

  1. Facultad de Ciencias Agrarias, Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), CONICET, Universidad Nacional de Rosario, Parque Villarino, S2125ZAA, Provincia de Santa Fe, Zavalla, Argentina

    F. I. Pozzi & S. A. Felitti

  2. Cátedra de Microbiología, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Parque Villarino, S2125ZAA, Provincia de Santa Fe, Zavalla, Argentina

    F. I. Pozzi

  3. Facultad de Ciencias Agrarias, Instituto de Botánica del Nordeste (IBONE), CONICET, Universidad Nacional del Nordeste, Sargento Cabral 2131, W3402BKG, Corrientes, Argentina

    C. A. Acuña

  4. Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sargento Cabral 2131, W3402BKG, Corrientes, Argentina

    C. L. Quarin

  5. Laboratorio de Biodiversidad Vegetal y Microbiana, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Parque Experimental Villarino, S2125ZAA, Provincia de Santa Fe, Zavalla, Argentina

    S. A. Felitti

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  1. F. I. Pozzi
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Pozzi, F.I., Acuña, C.A., Quarin, C.L. et al. GG13: a candidate gene related to seed development and viability from apomictic Paspalum notatum. Euphytica 217, 148 (2021). https://doi.org/10.1007/s10681-021-02881-1

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