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Mapping QTLs controlling low-temperature germinability in rice by using single segment substitution lines derived from 4 AA-genome species of wild rice

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Abstract

Rice growth is sensitive to low temperature. Chilling injury is one of the main natural disasters in rice cultivation, and is damaging to rice growth at all developmental stages. Low-temperature germinability (LTG) is an important trait for seedling establishment, especially in rice direct-sowing practice. However, the genetic mechanism of LTG remains elusive. Here, we report the mapping of QTLs controlling LTG in rice using 586 single segment substitution lines (SSSL) derived from 4 AA-genome wild rice species. 21 SSSLs with the highest germination rate at low temperature in both of the two tested seasons were selected, from them 10 QTLs controlling LTG were identified by substitution mapping to locate on 6 chromosomes: chromosome 1, 3, 5, 7, 8 and 11, with an interval length of 3.45 to 20.15 cM. Five QTLs were detected from Niv-SSSls: qLTGn3-1, qLTGn5-1, qLTGn5-3, qLTGn7-1 and qLTGn7-2, their additive effects ranged from 3.72% to 19.74% in late season 2018. qLTGb1-1 and qLTGb8-2 were detected in the SSSLs with O. barthii as the donor and they accounted for at least 9.51% of the total phenotypic variation. Two QTLs, qLTGg5-2 and qLTGg8-1, were derived from O. glumaepatula, and their average additive effects were 4.28% and 20.39% in late seasons 2018, respectively. qLTGm11-1 on chromosome 11 was the only QTL identified from O. meridionalis. All QTLs derived from wild rice species improved low-temperature germination significantly. The SSSL library with wild rice species as donor is therefore a valuable resource for rice germplasm innovation and the breeding of chilling-resistant varieties.

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Abbreviations

Chr.:

Chromosome

DDW:

Sterile distilled water

GR:

Germination rate

LTG:

Low-temperature germinability

MAS:

Marker-assisted selection

NIL:

Near-isogenic line

PCR:

Polymerase chain reaction

QTL:

Quantitative trait loci

RIL:

Recombinant inbred line

SSSL:

Single segment substitution line

SSR:

Simple sequence repeat

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Acknowledgements

We thank Zhe Ji (Department of Plant Sciences, University of Oxford) for suggestions.

Funding

This work was funded by the National Key Research and Development Program of China (2016YFD0100406), the Special Support Program of Guangdong Province for High-level Talents (2016TX03N224), and the National Natural Science Fund for Outstanding Young Science Foundation Project (31622041), and the State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources (SKLCUSA-a201907, -a201918).

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  1. Guangdong Provincial Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China

    Ruiqin Pei, Zhengao Zhang, Mingchuan Huang, Guangshan Hou, Jijing Luo, Haitao Zhu, GuiFu Liu, Xuelin Fu, Guiquan Zhang & Shaokui Wang

  2. College of Life Science and Technology, Guangxi University, Nanning, 530004, China

    Jijing Luo

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  1. Ruiqin Pei
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Contributions

S.W. conceived and designed the research. R.P., Z.Z., M.H. and G.H. carried out the experiments. H.Z. and G.L. analyzed the data. J. Land G.Z. provided suggestions and assistance for all the experiments. S.K. and R.P. wrote the manuscript. All of the authors edited and approved the final version of the manuscript.

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Correspondence to Shaokui Wang.

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Pei, R., Zhang, Z., Huang, M. et al. Mapping QTLs controlling low-temperature germinability in rice by using single segment substitution lines derived from 4 AA-genome species of wild rice. Euphytica 217, 58 (2021). https://doi.org/10.1007/s10681-021-02791-2

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