Skip to main content
SpringerLink
Log in

Mapping and DNA marker development for Lr33 from the leaf rust resistant line KU168-2

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

A wheat accession from the Kyoto University Collection, KU168-2, was identified as carrying good resistance to leaf rust and stem rust. To analyze this resistance a doubled haploid (DH) population was produced from hybrids of the cross KU168-2 with the leaf rust susceptible line RL6071. The DH population, of 116 lines, was evaluated for leaf rust resistance in the greenhouse at the seedling and adult plant stages, and in three field environments. Significant effects for reduction in leaf rust severity in the field were found on chromosome 1BL, most likely Lr33, which was also identified in the seedling tests, and on 7DS, which was identified as Lr34 in greenhouse adult plant tests and confirmed with a predictive molecular marker. In field trials, both Lr33 and Lr34 reduced the severity of leaf rust, while the lines with both resistance genes showed additivity of resistance. Similar results were consistently found from 2003 to 2017 testing Thatcher near-isogenic lines with Lr33, Lr34, and Lr33 + Lr34. A genetic map of the Lr33 region was developed and linked SNP markers were converted to KASP markers for the selection of Lr33 in wheat breeding programs. Two additional seedling resistance genes were found, one originating from KU168-2 on 6AL, and one from RL6071 on 7BL, but these genes did not contribute to field resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang SY, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420

    Article  CAS  PubMed  Google Scholar 

  • Allen AM, Barker GLA, Berry ST, Coghill JA, Gwilliam R, Kirby S, Robinson P, Brenchley RC, D’Amore R, McKenzie N, Waite D, Hall A, Bevan M, Hall N, Edwards KJ (2011) Transcript-specific, single-nucleotide polymorphism discovery and linkage analysis in hexaploid bread wheat (Triticum aestivum L.). Plant Biotechnol J 9:1086–1099

    Article  CAS  PubMed  Google Scholar 

  • Brennan PS (2010) Sustainable wheat rust resistance-Learning from history. A review for the Global Partnership Initiative for Plant Breeding Capacity Building (GIPB). FAO, Rome

  • Cui F, Zhang N, Fan XL, Zhang W, Zhao CH, Yang LJ, Pan RQ, Chen M, Han J, Zhao XQ, Ji J, Tong YP, Zhang HX, Jia JZ, Zhao GY, Li JM (2017) Utilization of a Wheat 660 K SNP array-derived high-density genetic map for high-resolution mapping of a major QTL for kernel number. Sci Rep 7:3788. https://doi.org/10.1038/s41598-017-04028-6

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Drijepondt SC, Pretorius ZA (1991) Expression of two wheat leaf rust resistance gene combinations involving Lr34. Plant Dis 75:526–528

    Article  Google Scholar 

  • Dyck PL (1977) Genetics of leaf rust reaction in three introductions of common wheat. Can J Genet Cytol 19:711–716

    Article  Google Scholar 

  • Dyck PL (1987) The association of a gene for leaf rust resistance with chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29:467–469

    Article  Google Scholar 

  • Dyck PL, Samborski DJ (1982) The inheritance of resistance to Puccinia recondita in a group of common wheat cultivars. Can J Genet Cytol 24:273–283

    Article  Google Scholar 

  • Dyck PL, Sykes EE (1994) Genetics of leaf-rust resistance in three spelt wheats. Can J Plant Sci 74:231–233

    Article  Google Scholar 

  • Dyck PL, Samborski DJ, Anderson RG (1966) Inheritance of adult-plant leaf rust resistance derived from the common wheat varieties ‘Exchange’ and ‘Frontana’. Can J Genet Cytol 8:665–671

    Article  Google Scholar 

  • Dyck PL, Kerber ER, Lukow OM (1987) Chromosome location and linkage of a new gene (Lr33) for reaction to Puccinia recondite f. sp. Tritici. Genome 29:463–466

    Article  Google Scholar 

  • Ellis JG, Lagudah ES, Spieleyer W, Dodds PN (2014) The past, present and future of breeding rust resistant wheat. Front Plant Sci 5:641

    Article  PubMed  PubMed Central  Google Scholar 

  • Ezzahiri B, Roelfs AP (1989) Inheritance and expression of adult plant resistance to leaf rust in ‘Era’ wheat. Plant Dis 7:549–551

    Article  Google Scholar 

  • Goutam U, Kukreja S, Yadav R, Salaria N, Thakur K, Goyal A (2015) Recent trends and perspectives of molecular markers against fungal diseases in wheat. Front Microbiol 6:861

    Article  PubMed  PubMed Central  Google Scholar 

  • Helguera M, Vanzetti L, Soria M, Khan IA, Kolmer J, Dubcovsky J (2005) PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines. Crop Sci 45:728–734

    Article  CAS  Google Scholar 

  • Herrera-Foessel SA, Singh RP, Huerta-Espino J, William HM, Garcia V, Djurle A, Yuen J (2008) Identification and molecular characterization of leaf rust resistance gene Lr14a in durum wheat. Plant Dis 92:469–473

    Article  CAS  Google Scholar 

  • Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L, Calvo-Salazar V, Lan C, Lagudah ES (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124:1475–1486

    Article  CAS  PubMed  Google Scholar 

  • Huerta-Espino J, Singh RP, Germán S, McCallum BD, Park RF, Chen WQ, Bhardwaj SC, Goyeau H (2011) Global status of wheat leaf rust caused by Puccinia triticina. Euphytica 179:143–160

    Article  Google Scholar 

  • Kassa MT, You FM, Fetch TG, Fobert P, Sharpe A, Pozniak CJ, Menzies JG, Jordan MC, Humphreys G, Zhu T, Luo MC, McCartney CA, Hiebert CW (2016) Genetic mapping of SrCad and SNP marker development for marker-assisted selection of Ug99 stem rust resistance in wheat. Theor Appl Genet 129:1373–1382

    Article  CAS  PubMed  Google Scholar 

  • Kolmer JA (2015) A QTL on chromosome 5BL in wheat enhances leaf rust resistance of Lr46. Mol Breed 35:74

    Article  Google Scholar 

  • Kolmer JA, Dyck PL, Roelfs AP (1991) An appraisal of stem and leaf rust resistance in North American hard red spring wheats and the probability of multiple mutations to virulence in populations of cereal rust fungi. Phytopathology 81:237–239

    Google Scholar 

  • Kosambi DD (1943) The estimation of map distances from recombination values. Ann Hum Genet 12:172–175

    Google Scholar 

  • Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown- Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898

    Article  CAS  PubMed  Google Scholar 

  • Liu RH, Meng JL (2003) MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Heraditas (Beijing) 25:317–321

    Google Scholar 

  • Long DL, Kolmer JA (1989) A North American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology 79:525–529

    Article  Google Scholar 

  • Lorieux M (2012) MapDisto: fast and efficient computation of genetic linkage maps. Mol Breed 30:1231–1235

    Article  CAS  Google Scholar 

  • Mago R, Miah H, Lawrence GJ, Wellings CR, Spielmeyer W, Bariana HS, McIntosh RA, Pryor AJ, Ellis JG (2005) High-resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome 1. Theor Appl Genet 112:41–50

    Article  CAS  PubMed  Google Scholar 

  • McCallum BD, Hiebert CW (2012) The interaction of the wheat multi-pest resistance gene Lr67 with other leaf rust resistance genes. In: Proceedings of the international plant and animal genome conference XX; 2012 Jan 14–18. San Diego (CA)

  • McCallum BD, Seto-Goh P (2003) Physiologic specialization of wheat leaf rust (Puccinia triticina) in Canada in 2000. Can J Plant Pathol 25:91–97

    Article  Google Scholar 

  • McCallum BD, Seto-Goh P, Xue A (2010) Physiological specialization of Puccinia triticina in Canada in 2007. Can J Plant Pathol 32:229–236

    Article  Google Scholar 

  • McCallum BD, Hiebert CW, Cloutier S, Bakkeren G, Rosa SB, Humphreys DG, Marais GF, McCartney CA, Panwar V, Rampitsch C, Saville BJ, Wang XB (2016) A review of wheat leaf rust research and the development of resistant cultivars in Canada. Can J Plant Pathol 38:1–18

    Article  CAS  Google Scholar 

  • McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Anderson OD (2005) Catalogue of gene symbols for wheat: 2005 supplement. http://shigen.lab.nig.ac.jp/wheat/komugi/top/top.jsp. Accessed 1 June 2018

  • Mundt CC (1991) Probability of mutation to multiple virulence and durability of resistance gene pyramids: further comments. Phytopathology 81:240–242

    Article  Google Scholar 

  • Mundt CC (2014) Durable resistance: a key to sustainable management of pathogens and pests. Infect Genet Evol 27:446–455

    Article  PubMed  Google Scholar 

  • Pederson ZA, Leath S (1988) Pyramiding major genes for resistance to maintain residual effects. Annu Rev Phytopathol 26:369–378

    Article  Google Scholar 

  • Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500

    Article  Google Scholar 

  • Ramirez-Gonzalez RH, Segovia V, Bird N, Fenwick P, Holdgate S, Berry S, Jack P, Caccamo M, Uauy C (2015) RNA-Seq bulked segregant analysis enables the identification of high-resolution genetic markers for breeding in hexaploid wheat. Plant Biotechnol J 13:613–624

    Article  CAS  PubMed  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  PubMed Central  Google Scholar 

  • Roelfs AP (1988) Resistance to leaf and stem rust in wheat. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico DF, pp 10–22

    Google Scholar 

  • Rosewarne GM, Singh RP, Huerta-Espino J, William HM, Bouchet S, Cloutier S, McFadden H, Lagudah ES (2006) Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theor Appl Genet 112:500–508

    Article  CAS  PubMed  Google Scholar 

  • Samborski DJ, Dyck PL (1982) Enhancement of resistance to Puccinia recondite by interaction of resistance genes in wheat. Can J Plant Pathol 4:152–156

    Article  Google Scholar 

  • Singh RP, McIntosh RA (1984) Complementary genes for reaction to Puccinia recondite tritici in Triticum aestivum L. Genetic and linkage studies. Can J Genet Cytol 26:723–735

    Article  Google Scholar 

  • Singh BD, Singh AK (2015) Marker-assisted plant breeding: principles and practices. Springer, Berlin

    Book  Google Scholar 

  • Singh RP, Mujeeb-Kazi A, Huerta-Espino J (1998) Lr46: a gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathology 88:890–894

    Article  CAS  PubMed  Google Scholar 

  • Singh D, Mohler V, Park RF (2013) Discovery, characterisation and mapping of wheat leaf rust resistance gene Lr71. Euphytica 190:131–136

    Article  CAS  Google Scholar 

  • Singla J, Luthi L, Wicker T, Bansal U, Krattinger SG, Keller B (2017) Characterization of Lr75: a partial, broad-spectrum leaf rust resistance gene in wheat. Theor Appl Genet 130:1–12

    Article  CAS  PubMed  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560

    Article  CAS  PubMed  Google Scholar 

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25

    Article  CAS  PubMed  Google Scholar 

  • Stakman EC, Stewart DM, Loegering WQ (1962) Identification of physiologic races of Puccinia graminis var. tritici. USDA agricultural research service E617, Washington DC

  • Tanaka M (1983) Catalogue of Aegilops-Triticum germ-plasm preserved in Kyoto University. Plant Germ-Plasm Institute, Faculty of Agriculture, Kyoto University, Kyoto Japan, p 179

  • Wang S, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, Lillemo M, Mather D, Appels R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo MC, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden M, Akhunov E (2014) Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhao XL, Zheng TC, Xia XC, He ZH, Liu DQ, Yang WX, Yin GH, Li ZF (2008) Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 117:1069–1075

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We would like to thank Elsa Reimer, Winnie McNabb, Mira Popovic, Ghassan Mardli, Suzanne Enns, Leslie Bezte and Devin Edwards for technical assistance. This study was supported by the “Building High Level University Program”/“MOE-AAFC Research Fellowship Program” authorised by China Scholarship Council, AAFC and China Agricultural University.

Author information

Authors and Affiliations

  1. Department of Plant Pathology, China Agricultural University, Beijing, 100193, People’s Republic of China

    Mingzhe Che & Zhongjun Zhang

  2. Morden Research and Development Centre, Agriculture and Agri-Food Canada, 101 Route 100, Unit 100, Morden, MB, R6M 1Y5, Canada

    Colin W. Hiebert, Curt A. McCartney & Brent D. McCallum

Authors
  1. Mingzhe Che
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Colin W. Hiebert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Curt A. McCartney
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhongjun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Brent D. McCallum
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

MC, CWH, CAM, BDM conceived, designed and managed the experiments. MC evaluated the population, contributed to the construction of the genetic maps, analyzed the data, wrote the first draft, and revised the manuscript. BDM evaluated the populations, analyzed the data, and revised the manuscript. CWH constructed the genetic maps, developed populations and KASP markers, and revised the manuscript. CAM performed the statistical and QTL analyses, and revised the manuscript. BDM, CWH, CAM and ZZ contributed to the grant proposals.

Corresponding author

Correspondence to Brent D. McCallum.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interests.

Statement of human and animal rights

This study did not include human or animal subjects.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Che, M., Hiebert, C.W., McCartney, C.A. et al. Mapping and DNA marker development for Lr33 from the leaf rust resistant line KU168-2. Euphytica 215, 29 (2019). https://doi.org/10.1007/s10681-019-2343-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10681-019-2343-3

Keywords

Search

Navigation