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Genetic investigation and cytological comparison of two genic male sterile lines 9012A and MSL in Brassica napus L.

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Abstract

Genic male sterility (GMS) is one of the effective pollination control systems in hybrid rapeseed (Brassica napus L.) breeding. The recessive GMS lines 9012A and Lembke (MSL) have made a great contribution to the utilization of heterosis in rapeseed. Cytological observation and genetic investigation were employed to reveal anther abortion characters and genetic relationship of 9012A and MSL. Cytological and transmission electron microscopy observation revealed that anther abortion of 9012A and MSL initiated at the microsporocyte stage, both lines showed premature or retarded degradation of tapetum. Some anthers in 9012A developed to the microspore stage since their tapetal cells changed to the secretory type. However, MSL anthers did not progress to the tetrad stage, since the tapetum did not change to the secretory type. Genetic investigation revealed that MSL and 9012A had the same temporary maintainer system, while their cytoplasm types were different. The cytoplasm of MSL was similar to Cam while 9012A was similar to Nap type. The results laid a solid foundation for utilization of the two male sterile lines in rapeseed hybrid breeding.

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Acknowledgements

We thank Dr. Miroslav Klima for kindly providing the hybrid rapeseed Marthow, Xiaohua He for technical assistance. This work was supported by the earmarked fund for China Agriculture Research System (CARS-13), and Tang Zhongyin breeding foundation of Northwest A&F University. The authors are grateful to anonymous reviewers for critical reading of the manuscript and their valuable suggestions.

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  1. Cancan Luo and Yanyan Sun have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Cancan Luo, Yanyan Sun, Yunxiao Zhang, Yuan Guo & Shengwu Hu

  2. Crop Research Institute, Drnovska 507, 16106, Prague 6, Czech Republic

    Miroslav Klima

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  1. Cancan Luo
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  2. Yanyan Sun
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  3. Yunxiao Zhang
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  4. Yuan Guo
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  5. Miroslav Klima
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Contributions

SH conceived and designed research. CL performed the experiments. CL and SH analyzed the data. CL, YS and SH wrote the manuscript. SH, CL, YZ, YG and MK discussed and edited the manuscript. All the authors read and approved the manuscript.

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Correspondence to Shengwu Hu.

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Luo, C., Sun, Y., Zhang, Y. et al. Genetic investigation and cytological comparison of two genic male sterile lines 9012A and MSL in Brassica napus L.. Euphytica 214, 124 (2018). https://doi.org/10.1007/s10681-018-2207-2

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