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Mapping quantitative trait loci for tolerance to phosphorus-deficiency at the seedling stage in barley

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Abstract

Phosphorus (P) deficiency in soil is a major factor that limits barley yield production. Increasing the tolerance to P-deficiency of barley is one of the most cost-effective solutions. Quantitative trait loci (QTLs) controlling P acquisition, P utilization efficiency and biomass at the seedling stage were identified using a population of recombinant inbred lines (RILs) subjected to two P concentrations (low P (LP), 25 µM and normal P (NP), 250 µM). The population was derived from a cross between Baudin and CN4027, which is a Hordeum spontaneum accession. In two hydroponic trials conducted in 2014 and 2016, seventeen QTLs were detected on chromosomes 2H, 3H, 4H and 5H at the two P concentrations. Eight of these QTLs influenced P acquisition efficiency (PAE). Phenotypic variation explained by a particular PAE-related QTL ranged from 13.3 to 39.9%. One QTL designated as Qspue.sau-3H.01 was related to P utilization efficiency (PUE); the phenotypic variation explained by this QTL was 12.5% (NP concentration) and 13.1% (LP concentration), respectively. Strong associations were observed between biomass and P efficiency-related traits in our study. Two QTL clusters controlling biomass, PAE- and PUE-related traits simultaneously were stably identified in the intervals bPb3263664–bPb3931069 and bPb3264570–bPb4786261 on chromosome 3H at both P concentrations in both trials. The QTLs related to PAE, PUE and biomass are important for the P-tolerant phenotype and may offer valuable clues for fine mapping and map-based cloning of barley.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31401377), Sichuan Science and Technology Support Project (2014NZ0008) and Project of Education Department in Sichuan Province (14ZA0002).

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  1. Jingyi Guo and Guangdeng Chen contributed equally to this publication.

Authors and Affiliations

  1. College of Resources, Sichuan Agricultural University, Chengdu, 611130, China

    Jingyi Guo, Guangdeng Chen, Xizhou Zhang, Tingxuan Li & Haiying Yu

  2. Faculty of Agriculture, Life and Environmental Sciences, University of Alberta, Edmonton, AB, T6G 2H4, Canada

    Hua Chen

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  1. Jingyi Guo
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Correspondence to Xizhou Zhang.

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Guo, J., Chen, G., Zhang, X. et al. Mapping quantitative trait loci for tolerance to phosphorus-deficiency at the seedling stage in barley. Euphytica 213, 114 (2017). https://doi.org/10.1007/s10681-017-1907-3

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