Skip to main content

Advertisement

SpringerLink
Log in

Identification of quantitative trait locus of zinc and phosphorus density in wheat (Triticum aestivum L.) grain

  • Regular Article
  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

Zinc (Zn) is an essential micronutrient for human beings. However, Zn malnutrition has become a major problem throughout the world. Wheat is the most important food crop in the world, therefore, developing Zn-enriched wheat varieties provides an effective approach to reduce Zn malnutrition in human beings. The aim of this study was to understand the genetic control of grain Zn density in wheat and hence, to provide genetic basis for breeding wheat with high grain Zn density using molecular approach. A doubled haploid (DH) population developed from a cross between winter wheat varieties Hanxuan10 and Lumai 14 was used to identify quantitative trait loci (QTLs) for Zn concentration and content in wheat grains. In addition, phosphorus (P) concentration and content in wheat grain were also investigated to examine possible interactions between these two nutrients. The wheat grains used in this study were harvested from the plants grown under normal condition in a field trial. We found the grain Zn concentrations of the DH population varied from 25.9 to 50.5 mg/kg and the Zn content varied from 0.90 to 2.21 μg/seed. The grain P concentrations of the DH population varied from 0.258 to 0.429 mg/kg, and the P contents varied from 0.083 to 0.186 mg/seed. A significant positive correlation was observed between Zn and P density in this experiment. The results showed that both grain Zn and P densities were controlled by polygenes. Four and seven QTLs for Zn concentration and Zn content were detected, respectively. All the four QTLs for Zn concentration co-located with the QTLs for Zn content, suggesting a possibility to improve both grain Zn concentration and content simultaneously. Four and six QTLs for P concentration and P content were detected, respectively. The two QTLs for grain Zn concentration on chromosomes 4A and 4D co-located with the QTLs for P concentration. The four QTLs for grain Zn content on chromosome 2D, 3A and 4A co-located with the QTLs for P contents, reflecting the positive correlations between the grain Zn and P density, and providing possibility of improving grain micro- and macronutrient density simultaneously in wheat. In order to improve human health, the effect of P–Zn relation in grain on the Zn bioavailability should also be considered in the future work.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • An DG, Su JY, Liu QY, Zhu YG, Tong YP, Li JM, Jing RL, Li B, Li ZS (2006) Mapping QTLs for nitrogen uptake in relation to the early growth of wheat (Triticum aestivum L.). Plant Soil 284:73–84

    Article  CAS  Google Scholar 

  • Basten CJ, Weir BS, Zeng ZB (2001) QTL CARTOGRAPHER, Version 1.15. Department of Statistics, North Carolina State University, Raleigh

  • Bouis HE (2002) Plant breeding: a new tool for fighting micronutrient malnutrition. J Nutr 132:491S–494S

    PubMed  CAS  Google Scholar 

  • Cakmak I, Torun A, Miller E, Feldman M, Fahima T, Korol A, Nevo E, Braun HJ, özkan H (2004) Triticum dicoccoides: an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat. Soil Sci Plant Nutr 50:1047–1054

    CAS  Google Scholar 

  • Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142:169–196

    Article  CAS  Google Scholar 

  • Erdal I, Yilmaz A, Taban S, Eker S, Torun B, Cakmak I (2002) Phytic acid and phosphorus concentration in seeds of wheat cultivars grown with and without zinc fertilization. J Plant Nutr 25:113–127

    Article  CAS  Google Scholar 

  • Fagerial VD (2001) Nutrient interactions in crop plants. J Plant Nutr 24:1269–1290

    Article  Google Scholar 

  • Frossard E, Bucher M, Machler F, Mozafar A, Hurrell R (2000) Potential for increasing the content and bioavailability of Fe, Zn and Ca in plants for human nutrition. J Sci Food Agr 80:861–879

    Article  CAS  Google Scholar 

  • Ghandilyan A, Vreugdenhil D, Aarts MGM (2006) Progress in the genetic understanding of plant iron and zinc nutrition. Physiol Plantarum 126:407–417

    Article  CAS  Google Scholar 

  • Graham RD, Senadhira D, Beebe S, Iglesias C, Monasterio I (1999) Breeding for micronutrient density in edible portions of staple food crops: conventional approaches. Field Crop Res 60:57–80

    Article  Google Scholar 

  • Haldar M, Mandal LN (1981) Effect of phosphorus and zinc on the growth and phosphorus, zinc, copper, iron and manganese nutrition of rice. Plant Soil 59:415–425

    Article  CAS  Google Scholar 

  • Hao CY, Wang LF, Zhang XY, You GX, Dong YS, Jia JZ, Liu X, Shang XW, Liu SC, Cao YS (2005) Evolvement of genetic diversity in wheat varieties in China (in Chinese). Sci China Ser C-Life Sci 35:408–415

    Google Scholar 

  • Lan XX (2003) Zinc deficiency and children health (in Chinese). Chinese J Women Child Health Res 14:49–51

    Google Scholar 

  • Liang JF (2006) Study of contents of iron, zinc and phytic acid in rice and effect of processing on concentrations and solubility of iron and zinc (in Chinese). PhD thesis, China Agricultural University (Beijing)

  • Lonergan PF (2001) Genetic characterization of QTL mapping of zinc nutrition in barley (Hordeum vulgare L.) PhD thesis, University of Adelaide (Australia)

  • Mazzolini BAP, Pallaghy CK, Legge GJF (1985) Quantitative microanalysis of Mn, Zn and other elements in mature wheat seed. New Phytol 100:483–509

    Article  CAS  Google Scholar 

  • McDonald GK, Genc Y, Lewis J, Graham RD (2007) Relationships between zinc and other nutrients in wheat grain. Zinc Crops 2007-Posters

  • Morgounov A, Ferney H, Gomez-Becerra H, Abugalieva A, Dzhunusova M, Yessimbekova M, Muminjanov H, Zelenskiy Y, Ozturk L, Cakmak I (2007) Iron and zinc grain density in common wheat grown in central Asia. Euphytica 155:193–203

    Article  Google Scholar 

  • Moussavi-Nik M, Pearson JN, Hollamby GJ, Graham RD (1998) Dynamics of nutrient remobilization during germination and early seedling development in wheat. J Plant Nutr 21:421–434

    Article  CAS  Google Scholar 

  • Ozturk L, Yazici MA, Yucel C, Torun A, Cekic C, Bagci A, Ozkan H, Braun HJ, Sayers Z, Cakmak I (2006) Concentration and localization of zinc during seed development and germination in wheat. Physiol. Plantarum 128:144–152

    Article  CAS  Google Scholar 

  • Peterson CJ, Johnson VA, Mattern PJ (1986) Influence of cultivar and environment on mineral. Cereal Chem 63:183–186

    CAS  Google Scholar 

  • Raboy V (2000) Low-phytic-acid grains. Food Nutr Bull 21:423–427

    Google Scholar 

  • Rengel Z, Batten GD, Crowley DE (1999) Agronomic approaches for improving the micronutrient density in edible portions of field crops. Field Crops Res 60:29–40

    Article  Google Scholar 

  • Rodriguez MA, Gustafson JP (2001) Genetic and physical characterization of chromosome 4DL in wheat. Genome 44:883–892

    Article  Google Scholar 

  • Stangoulis JCR, Huynh BL, Welch RM, Choi EY, Graham RD (2006) Quantitative trait loci for phytate in rice grain and their relationship with grain micronutrient content. Euphytica 0014-2336 (Print) 1573-5060 (Online)

  • Torun A, GÜltekin M, Kalayci M, Yilmaz A, Eker S, Cakmak I (2001) Effect of zinc fertilization on grain yield and shoot concentrations of zinc, boron, and phosphorus of 25 wheat cultivars grown on a zinc-deficient and boron-toxic soil. J Plant Nutr 24:1817–1829

    Article  CAS  Google Scholar 

  • Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314:1298–1301

    Article  PubMed  CAS  Google Scholar 

  • Vreugdenhil D, Aarts MGM, Koornneef M, Nelissen H, Ernst WHO (2004) Natural variation and QTL analysis for cationic mineral content in seeds of Arabidopsis thaliana. Plant Cell Environ 27:828–839

    Article  CAS  Google Scholar 

  • Wakaki J, Yonetani R, Kuroda S, Shinano T, Yazaki J, Fujii F, Shimbo K, Yamamoto K, Sakata K, Kishimoto N, Kikuchi S, Yamagishi M, Osaki M (2003) Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots. Plant Cell Environ 26:1515–1523

    Article  Google Scholar 

  • Welch RM, Graham RD (2004) Breeding for micronutrients in staple food crops from a human nutrition perspective. J Exp Bot 55:353–364

    Article  PubMed  CAS  Google Scholar 

  • Welch RM, Graham RD (2005) Agriculture: the real nexus for enhancing bioavailable micronutrients in food crops. J Trace Elem Med Biol 18:299–307

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 30671239), the Program for Changjiang Scholars and Innovative Research Team in University (IRT0511), and Chinese Academy of Sciences (Grant No. KSCX2-YW-N-056). The authors would like to thank Dr. Junying Su for the assistance in statistical analysis of the data. The authors are also grateful to Dr. Graham Lyons in Adelaide University of Australia and Dr. Min Du in USA for their helpful comments on the manuscript.

Author information

Authors and Affiliations

  1. Department of Plant Nutrition, China Agricultural University, 2 West Yuanmingyuan Road, Haidian District, Beijing, 100094, China

    Rongli Shi, Fusuo Zhang & Chunqin Zou

  2. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Hongwei Li & Yiping Tong

  3. Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    Ruilian Jing

Authors
  1. Rongli Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hongwei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yiping Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ruilian Jing
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fusuo Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chunqin Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chunqin Zou.

Additional information

Responsible Editor: Ismail Cakmak.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shi, R., Li, H., Tong, Y. et al. Identification of quantitative trait locus of zinc and phosphorus density in wheat (Triticum aestivum L.) grain. Plant Soil 306, 95–104 (2008). https://doi.org/10.1007/s11104-007-9483-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11104-007-9483-2

Keywords

Search

Navigation