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Identification of the target region including the Foc0 1 /foc0 1 gene and development of near isogenic lines for resistance to Fusarium Wilt race 0 in chickpea

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Abstract

Three recombinant inbred populations (RIP-1, RIP-7 and RIP-10) were used to validate and saturate the target area involved in the resistance to Fusarium oxysporum f.sp. ciceris race 0 (Foc0) in chickpeas. With the aim of physically localizing the genetic area controlling Foc0 resistance, a new genetic map of the linkage group 5 (LG5) and a physical map of the Ca5 pseudomolecule were drawn up and compared. The genetic and physical between CaGM20820 and CaGM20889 (16759541–17501349 bp) within an interval of 2 cM comprising 27 annotated genes. Only two of the annotated genes in this region could mapping results obtained were useful to refine the position of the target area be involved in resistance mechanisms. The three RIPs were used to develop three pairs of near isogenic lines (NILs) for resistance/susceptibility to Foc0. NILs are a valuable tool for fine mapping, refining the target region and selecting candidate genes for resistance to Foc0. The information reported in this paper will help develop diagnostic markers, considered useful tools for MAS in chickpea breeding programs.

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Acknowledgments

We wish to thank all of the technical staff who contributed to this work from the laboratory of genetics of the University of Cordoba and from the Tunisian Chickpea Program. W. Jendoubi was supported by a fellowship from the MESRT (Ministère de l’Enseignement Supérieur et de la Recherche Scientifique-Tunisie) and the Tunisian Project on Chickpeas (Efficacité de la sélection assistée par marqueurs dans la détection de génotypes de pois chiche résistants aux principales maladies fongiques en Tunisie) funded by IRESA (Institution de la Recherche et de l’Enseignement Supérieur Agricole- Ministère de l’Agriculture).

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  1. E. Madrid

    Present address: Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany

Authors and Affiliations

  1. National Agronomic Institute of Tunisia, 43 Av Charles Nicolle, 1082, Tunis, Tunisia

    W. Jendoubi

  2. Field Crop Laboratory, National Institute for Agricultural Research of Tunisia (INRAT), University of Carthage, Rue Hédi Karray, 2080, Tunis, Tunisia

    W. Jendoubi, M. Bouhadida & M. Kharrat

  3. Departamento de Genética, University of Córdoba, Campus Rabanales, Edif. C5, 14071, Córdoba, Spain

    T. Millan & J. Gil

  4. Área de Mejora y Biotecnología, IFAPA Centro “Alameda del Obispo”, Apdo 3092, 14080, Córdoba, Spain

    J. Rubio

  5. Institute for Sustainable Agriculture, CSIC, Apdo 4084, 14080, Cordoba, Spain

    E. Madrid

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Jendoubi, W., Bouhadida, M., Millan, T. et al. Identification of the target region including the Foc0 1 /foc0 1 gene and development of near isogenic lines for resistance to Fusarium Wilt race 0 in chickpea. Euphytica 210, 119–133 (2016). https://doi.org/10.1007/s10681-016-1712-4

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