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Genetic analysis and molecular mapping of Pitb with broad-spectrum resistance to Magnaporthe oryzae in rice

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Abstract

Zixuan 2 is a broad spectrum-resistance indica subspecies (Oryza sativa ssp. indica) with a high resistance to 15 Magnaporthe oryzae strains from ZA, ZB, and ZC. The F1 generation of hybrid between Zixuan 2 and Lijiangxintuanheigu showed significant resistance to rice-blast pathogen ZB13, and the ratio of resistant plants to susceptible plants was 3:1, indicating that the resistance of Zixuan 2 to rice blast is controlled by a single dominant karyogene or a quantitative trait locus, designated here as Pitb. Molecular marker analysis showed that the rice blast-resistance gene is located on the terminal short arm of chromosome 12 between IN936 and IN950, with a genetic distance of 3.0 cM and physical distance of 143 kbp. In addition, the localization found that Pitb was coseparated with marker IN937. The genetic data and recombination populations provided in this study will enable further fine-mapping and cloning of the resistance gene.

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Acknowledgments

This research study was supported by grant from China Postdoctoral Science Foundation (No. 2015M571987), Jiangxi Province Postdoctoral Science Foundation (No. 2015KY24), Jiangxi Province Technology Hall (No. 20152ACF60011, No. 20112BBF60008) and Jiangxi Province education Hall (No. GJJ150763).

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Authors and Affiliations

  1. College of Life Science, Jinggangshan University, Ji’an, 343000, China

    Huimin Sun, Lihua Wu & Zhuo Zheng

  2. College of Life Science, Tongji University, Shanghai, 200092, China

    Huimin Sun & Zhou Cheng

  3. Ji’an City of Agricultural Sciences, Ji’an, 343000, China

    Longping Guo & Fugen Song

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  1. Huimin Sun
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  2. Lihua Wu
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  3. Longping Guo
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  5. Zhuo Zheng
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Correspondence to Zhuo Zheng.

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Sun, H., Wu, L., Guo, L. et al. Genetic analysis and molecular mapping of Pitb with broad-spectrum resistance to Magnaporthe oryzae in rice. Euphytica 209, 547–554 (2016). https://doi.org/10.1007/s10681-015-1608-8

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  • DOI: https://doi.org/10.1007/s10681-015-1608-8

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