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DNA marker-assisted selection to pyramid rust resistance genes in “carioca” seeded common bean lines

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Abstract

This work reports a gene pyramiding approach assisted by DNA markers used to develop “carioca” seeded common bean (Phaseolus vulgaris L.) elite lines harboring three different rust resistance genes. Rust is among the most destructive diseases that attack P. vulgaris and cause serious damage worldwide. The rust resistance genes Ur-5 (from ‘Mexico 309’), Ur-11 (from ‘BelMiDak RR-3’), and Ur-14 (from ‘BRS Pioneiro’, a “carioca” seeded cultivar derived from the resistance source ‘Ouro Negro’) were combined in the “carioca” seeded bean cultivar ‘Rudá’. Firstly, two different backcross programs were conducted separately to produce progenies harboring individually the Ur-5 and Ur-11 genes. Molecular fingerprinting analysis was used to select plants genetically similar to ‘Rudá’ in the backcross cycles to accelerate the recurrent-background recovery. The obtained progenies were initially intercrossed and then crossed with ‘BRSMG Pioneiro’ (Ur-14). The final F1 plants derived from these crosses were screened with DNA markers linked to the three rust resistance genes: SI19 (Ur-5), SAE19 (Ur-11) and OPX11 (Ur-14). The plants selected as harboring all the alleles of interest were used to obtain the next generations. The selection based on DNA markers was conducted up to the F4:5 generation. We were able to select F4:7 progenies showing all the DNA markers associated to the genes of interest and resistant to all specific races of U. appendiculatus used for phenotypically detecting each one of the rust resistance genes. Yield evaluations show that these selected lines are as productive as the recurrent parent ‘Rudá’ and high-performing control cultivars grown in Brazil.

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Acknowledgments

This work was supported by grants from “Empresa Brasileira de Pesquisa Agropecuária—Embrapa”, “Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq” and “Fundação de Amparo à Pesquisa do Estado de Minas Gerais—FAPEMIG” (Brazilian Government). CNPq also provided a research fellowship to the first author, which was greatly appreciated.

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Authors and Affiliations

  1. Embrapa Arroz e Feijão (Embrapa Rice and Beans), GO-462, km 12, Santo Antônio de Goiás, GO, 75375-000, Brazil

    Thiago Lívio P. O. Souza

  2. Universidade Federal de Goiás (UFG), BR-364, km 192, Jataí, GO, 75800-000, Brazil

    Vilmar A. Ragagnin

  3. Embrapa Cerrados, BR-020, km 18, Planaltina, DF, 73310-970, Brazil

    Suelen N. Dessaune

  4. Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Agrárias (ICA), Montes Claros, MG, 39404-547, Brazil

    Demerson A. Sanglard

  5. Universidade Federal de Viçosa (UFV), Viçosa, MG, 36570-000, Brazil

    José Eustáquio S. Carneiro, Maurilio A. Moreira & Everaldo G. Barros

  6. Universidade Católica de Brasília, Pós-Graduação em Ciências Genômicas e Biotecnologia, Brasília, DF, 70790-160, Brazil

    Everaldo G. Barros

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  1. Thiago Lívio P. O. Souza
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  2. Vilmar A. Ragagnin
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  3. Suelen N. Dessaune
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  4. Demerson A. Sanglard
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  5. José Eustáquio S. Carneiro
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  6. Maurilio A. Moreira
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  7. Everaldo G. Barros
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Correspondence to Thiago Lívio P. O. Souza.

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Souza, T.L.P.O., Ragagnin, V.A., Dessaune, S.N. et al. DNA marker-assisted selection to pyramid rust resistance genes in “carioca” seeded common bean lines. Euphytica 199, 303–316 (2014). https://doi.org/10.1007/s10681-014-1126-0

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  • DOI: https://doi.org/10.1007/s10681-014-1126-0

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