Skip to main content

Advertisement

SpringerLink
Log in

Evaluation and QTL mapping of phosphorus concentration in soybean seed

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Phosphorus (P) is an essential macronutrient required for many biological and metabolic plant functions. Phosphate is the form of P used by plants. Reducing the amount of P in the seed can further aide breeding efforts to reduce the amount of P released into the environment due to the indigestibility of the complexes formed with metal ions. Analysis of the variation of phosphorus concentration in soybean seed under non-stressed conditions revealed that phosphorus ranged from 3,948.1 to 5,695.8 μg/g total phosphorous (TP) in combined years. The averages for independent years were significantly different from one another. Quantitative trait loci (QTL) analysis of TP was performed to identify candidate gene(s) that is (are) involved in P accumulation in soybean seed. One putative QTL region was identified on chromosome 12 in the combined data that contained a phosphate transporter gene. Two additional suggestive QTL were identified on chromosomes 7 and 17 with chromosome 7 having both a phosphate transport gene and a ZIP transporter gene in the region of the QTL. There were additional genes in these regions that are involved in phosphate metabolism and transport.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Anonymous (2009) JMP ~ 8: statistical discovery software

  • Bilyeu KD, Zeng P, Coello P, Zhang ZJ, Krishnan HB, Bailey A, Beuselinck PR, Polacco JC (2008) Quantitative conversion of phytate to inorganic phosphorus in soybean seeds expressing a bacterial phytase. Plant Physiol 146:468–477

    Article  PubMed  CAS  Google Scholar 

  • Blair MW, Sandoval TA, Caldas GV, Beebe SE, Páez MI (2009) Quantitative trait locus analysis of seed phosphorus and seed phytate content in a recombinant inbred line population of common bean. Crop Sci 49:237–246

    Article  CAS  Google Scholar 

  • Chiou TJ, Liu H, Harrison MJ (2001) The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface. Plant J 25:281–293

    Article  PubMed  CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Cichy KA, Caldas GV, Snapp SS, Blair MW (2009) QTL analysis of seed iron, zinc, and phosphorus levels in an Andean bean population. Crop Sci 49:1742–1750

    Article  CAS  Google Scholar 

  • Correll DL (1998) The role of phosphorus in the eutrophication of receiving waters: a review. J Environ Qual 27:261–266

    Article  CAS  Google Scholar 

  • de Cianzio SR, Fehr WR (1980) Genetic control of iron deficiency chlorosis in soybeans. Iowa State J Res 54:367–375

    Google Scholar 

  • Divecha N, Irvine RF (1995) Phospholipid signaling review. Cell 80:269–278

    Article  PubMed  CAS  Google Scholar 

  • Fehr WR (1987) Principles of cultivar development: theory and technique. Macmillan Publishing Company, New York

    Google Scholar 

  • Fehr WR, Voss BK, Cianzio S (1984) Registration of a germplasm line of soybean, A7. Crop Sci 24:390–391

    Article  Google Scholar 

  • Flugge U-I (1999) Phosphate translocators in plastids. Annu Rev Plant Physiol Plant Mol Biol 50:27–45

    Article  PubMed  CAS  Google Scholar 

  • Flügge UI, Fischer K, Gross A, Sebald W, Lottspeich F, Eckerskorn C (1989) The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts. Eur Mol Biol Organ J 8:39–46

    Google Scholar 

  • Fu J, Peterson K, Guttieri M, Souza E, Raboy V (2008) Barley (Hordeum vulgare L.) inositol monophosphatase: gene structure and enzyme characteristics. Plant Mol Biol 67:629–642

    Article  PubMed  CAS  Google Scholar 

  • Gao Y, Shang C, Maroof MAS, Biyashev RM, Grabau EA, Kwanyuen P, Burton JW, Buss GR (2007) A modified colorimetric method for phytic acid analysis in soybean. Crop Sci 47:1797–1803

    Article  CAS  Google Scholar 

  • Jansen RC (1993) Interval mapping of multiple quantitative trait loci. Genetics 135:205–211

    PubMed  CAS  Google Scholar 

  • Jansen RC (1994) Controlling the Type I and Type II errors in mapping quantitative trait loci. Genetics 138:871–881

    PubMed  CAS  Google Scholar 

  • King KE, Peiffer GA, Lauter N, Reddy M, Lin SF, Cianzio S, Shoemaker RC (2012) Mapping of iron and zinc quantitative trait loci in soybean (Glycine max (L.) Merr.) for association to iron deficiency chlorosis resistance. J Plant Nutr (in press)

  • Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    PubMed  CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Li Y-D, Wang Y-J, Tong Y-P, Gao J-G, Zhang J-S, Chen S-Y (2005) QTL mapping of phosphorus deficiency tolerance in soybean (Glycine max L. Merr.). Euphytica 142(1–2):137–142

    Article  CAS  Google Scholar 

  • Liang Q, Cheng X, Mei M, Yan X, Liao H (2010) QTL analysis of root traits as related to phosphorus efficiency in soybean. Ann Bot 106:223–234

    Article  PubMed  CAS  Google Scholar 

  • Lin S, Cianzio S, Shoemaker R (1997) Mapping genetic loci for iron deficiency chlorosis in soybean. Mol Breed 3:219–229

    Article  CAS  Google Scholar 

  • Liu B, Fujita T, Yan Z-H, Sakamoto S, Xu D, Abe J (2007) QTL mapping of domestication-related traits in soybean (Glycine max). Ann Bot 100:1027–1038

    Article  PubMed  CAS  Google Scholar 

  • Loewus FA, Loewus MW (1983) Myo-Inositol: its biosynthesis and metabolism. Ann Rev Plant Physiol 34:137–161

    Article  CAS  Google Scholar 

  • Lou Yi, Gou J-Y, Xue H-W (2007) PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth. Plant Cell 19:163–181

    Article  PubMed  CAS  Google Scholar 

  • Marschner H (1995). Mineral nutrition of higher plants, vol 2, Academic Press, San Diego

  • Maupin LM, Rosso ML, Shang C, Rainey KM (2011) Genotype × environment interaction and stability of phosphorus concentration in two soybean germplasm sources with modified phosphorus composition. Crop Sci 51:1518–1524

    Article  Google Scholar 

  • Oltmans SE, Fehr WR, Welke GA, Raboy V, Peterson KL (2005) Agronomic and seed traits of soybean lines with low-phytate phosphorus. Crop Sci 45:593–598

    Article  CAS  Google Scholar 

  • Pao SS, Paulsen IANT, Saier MH (1998) Major facilitator superfamily. Microbiology 62:1–34

    CAS  Google Scholar 

  • Paran I, Zamir D (2003) Quantitative traits in plants: beyond the QTL. Trends Genet 19:303–306

    Article  PubMed  CAS  Google Scholar 

  • Poirier Y, Bucher M (2002) Phosphate transport and homeostasis in Arabidopsis. The Arabidopsis Book 1:1–35

    Article  Google Scholar 

  • Price AH (2006) Believe it or not, QTLs are accurate! Trends Plant Sci 11:213–216

    Article  PubMed  CAS  Google Scholar 

  • Raboy V (1997) Accumulation and storage of phosphate and minerals. In: Larkins BA, Vasil IK (eds) Cellular and molecular biology of plant seed development, Kluwer Academic Publishers, Dordrecht, p 441–477

  • Raboy V (2001) Seeds for a better future: “low phytate” grains help to overcome malnutrition and reduce pollution. Trends Plant Sci 6:458–462

    Article  PubMed  CAS  Google Scholar 

  • Raboy V, Dickinson DB, Below FE (1984) Variation in seed total phosphorus, phytic acid, zinc, calcium, magnesium, and protein among lines of Glycine max and G. soja1. Crop Sci 24:431–434

    Article  CAS  Google Scholar 

  • Raboy V, Young KA, Dorsch JA, Cook AL (2001) Genetics and breeding of seed phosphorus and phytic acid. J Plant Physiol 158:489–497

    Article  CAS  Google Scholar 

  • Raghothama KG (1999) Phosphate acquisition. Ann Rev Plant Physiol Plant Mol Biol 50:665–693

    Article  CAS  Google Scholar 

  • Reddy NR, Pierson MD, Sathe SK, Salunkhe DK (1989) Phytates in cereals and legumes, CRC Press, Boca Raton

  • Schlemmer U, Müller H, Jany KD (1995) The degradation of phytic acid in legumes prepared by different methods. Eur J Clin Nutr 49(Suppl 3):S207–S210

    PubMed  Google Scholar 

  • Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL et al (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183

    Article  PubMed  CAS  Google Scholar 

  • Schulz B, Kolukisaoglu HU (2006) Genomics of plant ABC transporters: the alphabet of photosynthetic life forms or just holes in membranes? FEBS Lett 580:1010–1016

    Article  PubMed  CAS  Google Scholar 

  • Sharpley AN, Withers PJA (1994) The environmentally-sound management of agricultural phosphorus. Fertilizer Res 39:133–146

    Article  CAS  Google Scholar 

  • Smith FW, Mudge SR, Rae AL, Glassop D (2003) Phosphate transport in plants. Plant Soil 248:71–83

    Article  CAS  Google Scholar 

  • Song Qijian, Jia G, Zhu Y, Grant D, Nelson RT, Hwang E-Y, Hyten DL, Cregan PB (2010) Abundance of SSR motifs and development of candidate polymorphic SSR markers (BARCSOYSSR_1.0) in soybean. Crop Sci 50:1950–1960

    Article  CAS  Google Scholar 

  • Tatusov Roman L, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM et al (2003) The COG database: an updated version includes eukaryotes. BMC Bioinform 4:1–14

    Article  Google Scholar 

  • Tiwari VK, Rawat N, Chhuneja P, Neelam K, Aggarwal R, Randhawa GS, Dhaliwal HS, Keller B, Singh K (2009) Mapping of quantitative trait Loci for grain iron and zinc concentration in diploid A genome wheat. J Hered 100:771–776

    Article  PubMed  CAS  Google Scholar 

  • Tsao GT, Zheng Y, Lu J, Gong CS (1997) Adsorption of heavy metal ions by immobilized phytic acid. Appl Biochem Biotechnol 63–65:731–741

    Article  PubMed  Google Scholar 

  • van Ooijen, JW (2009) MapQTL 6: software for the mapping of quantitative trait loci in experimental populations of diploid species. Wageningen, The Netherlands. http://www.kyazma.nl/docs/MQ6Manual.pdf. Accessed 30 March 2011

  • Vreugdenhil D, Aarts MGM, Koornneef M, Nelissen H, Ernst WHO (2004) Natural variation and QTL analysis for cationic mineral content in seeds of Arabidopsis thaliana. Plant Cell Environ 27:828–839

    Article  CAS  Google Scholar 

  • Willems G, Frérot H, Gennen J, Salis P, Saumitou-Laprade P, Verbruggen N (2010) Quantitative trait loci analysis of mineral element concentrations in an Arabidopsis halleri × Arabidopsis lyrata petraea F2 progeny grown on cadmium-contaminated soil. New Phytol 187:368–379

    Article  PubMed  CAS  Google Scholar 

  • Wu Z, Zhao J, Gao R, Hu G, Gai J, Xu G, Xing H (2011) Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max. PLoS ONE 6:1–12

    Google Scholar 

  • Zheng L, Huang F, Narsai R, Wu J, Giraud E, He F, Cheng L et al (2009) Physiological and transcriptome analysis of iron and phosphorus interaction in rice seedlings. Plant Physiol 151:262–274

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge the financial support of the USDA-ARS and Iowa State University. Names are necessary to report factually on the available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by the USDA implies no approval of the product to the exclusion of others that may also be suitable. Additionally, The authors thank Jonathan Hobbs of the Iowa State University Agriculture Experiment Station Consulting Group for assistance with data imputation.

Author information

Authors and Affiliations

  1. Department of Agronomy, Iowa State University, Ames, IA, USA

    Keith E. King

  2. Corn Insects and Crop Genetics Research Unit, USDA-ARS, Ames, IA, USA

    Nick Lauter, M. Paul Scott & Randy C. Shoemaker

  3. Department of Agronomy, National Taiwan University, Taipei, Taiwan, ROC

    Shun Fu Lin

Authors
  1. Keith E. King
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nick Lauter
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shun Fu Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Paul Scott
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Randy C. Shoemaker
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Randy C. Shoemaker.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 2458 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

King, K.E., Lauter, N., Lin, S.F. et al. Evaluation and QTL mapping of phosphorus concentration in soybean seed. Euphytica 189, 261–269 (2013). https://doi.org/10.1007/s10681-012-0797-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-012-0797-7

Keywords

Search

Navigation