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Improved S-genotyping and new incompatibility groups in Japanese plum

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Abstract

The selection of cross-compatible cultivars is essential to ensure fruit set in self-incompatible species like Japanese plum and thus the S-genotype must be determined in order to establish incompatibility groups. In this study an improved Japanese plum S-genotyping method, based in polymerase chain reaction and capillary electrophoresis detection of intron polymorphisms of S-locus genes, S-RNase and SFB, has been assayed and validated in a wide sample of cultivars. This method allows a more precise determination of amplified fragment sizes and therefore a better differentiation of self-incompatibility alleles. The assayed methodology was proven effective in the detection of 13 different S-alleles of S-RNases and SFBs and was used to S-genotype 105 Japanese plum cultivars, 32 of which are described by first time in this work. Analysed cultivars were assigned into 11 incompatibility groups and two new incompatibility groups (XX and XXI) were identified, increasing to 21 the number of incompatibility groups described in this crop.

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Acknowledgments

We gratefully acknowledge J.L. Espada [Centro de Técnicas Agrarias (CTA-DGA)], M. Alonso [Cooperativa de Regantes de Extremadura (CREX)], I. Iglesias [Institut de Recerca i Tecnologia Agroalimentàries (IRTA-Generalitat de Catalunya)], J. R. Rituerto (RITUCON Consultoría Agronómica), Grupo ALM, Viveros Provedo, Viveros Atanasio Naranjo, Agroseguro S.A., USDA-ARS-National Clonal Germplasm Repository (Davis, California) and Dr. R. Tao (Kyoto University, Japan) for providing plant material used in this work. Financial support for this research was provided by the Spanish Ministry of Science and Innovation-European Regional Development Fund (ERDF), European Union(Project grants: AGL2009-12621-C02-02; NIA-RTA2009-000144), Government of Aragón (Grupo de Excelencia A-43) and M.E. Guerra was financed by an Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) doctoral fellowship.

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Authors and Affiliations

  1. Departmento de Hortofruticultura, Centro de Investigación Agraria ‘Finca La Orden-Valdesequera’, 06187, Guadajira, Badajoz, Spain

    María E. Guerra & Margarita López-Corrales

  2. Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Av. Montañana 930, 50059, Zaragoza, Spain

    Ana Wünsch

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  1. María E. Guerra
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  2. Margarita López-Corrales
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  3. Ana Wünsch
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Correspondence to Ana Wünsch.

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Guerra, M.E., López-Corrales, M. & Wünsch, A. Improved S-genotyping and new incompatibility groups in Japanese plum. Euphytica 186, 445–452 (2012). https://doi.org/10.1007/s10681-012-0636-x

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  • DOI: https://doi.org/10.1007/s10681-012-0636-x

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